6xia
From Proteopedia
(New page: 200px<br /><applet load="6xia" size="450" color="white" frame="true" align="right" spinBox="true" caption="6xia, resolution 1.65Å" /> '''REFINEMENT OF GLUCOS...) |
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| - | [[Image:6xia.gif|left|200px]]<br /><applet load="6xia" size=" | + | [[Image:6xia.gif|left|200px]]<br /><applet load="6xia" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="6xia, resolution 1.65Å" /> | caption="6xia, resolution 1.65Å" /> | ||
'''REFINEMENT OF GLUCOSE ISOMERASE FROM STREPTOMYCES ALBUS AT 1.65 ANGSTROMS WITH DATA FROM AN IMAGING PLATE'''<br /> | '''REFINEMENT OF GLUCOSE ISOMERASE FROM STREPTOMYCES ALBUS AT 1.65 ANGSTROMS WITH DATA FROM AN IMAGING PLATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of 'metal-free' glucose isomerase of Streptomyces albus | + | The structure of 'metal-free' glucose isomerase of Streptomyces albus strain number YT ATCC 21132 has been analysed and refined at 1.65 A. The space group is I222, with cell dimensions a = 93.9 (1), b = 99.7 (1) and c = 102.9 (1) A, and there is one monomer of the tetrameric molecule per asymmetric unit. The data were recorded from two crystals of the protein using synchrotron radiation from the EMBL beamline X11 at DESY, Hamburg. Data were recorded with an imaging plate scanner designed and built in the EMBL Hamburg outstation. The total data-collection time was less than 12 h and the processing of all data took less than 2 days. The coordinates of the Arthrobacter glucose isomerase refined at a resolution of 2.5 A were used as a starting model. The structure of the protein and of 445 associated water molecules in the asymmetric unit were refined by restrained least-squares minimization using all data between 8 and 1.65 A to a final R factor of 14.1%. |
==About this Structure== | ==About this Structure== | ||
| - | 6XIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_albus Streptomyces albus]. Active as [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] Full crystallographic information is available from [http:// | + | 6XIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_albus Streptomyces albus]. Active as [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XIA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
[[Category: Terry, H.]] | [[Category: Terry, H.]] | ||
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
[[Category: isomerase(intramolecular oxidoreductse)]] | [[Category: isomerase(intramolecular oxidoreductse)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:51 2008'' |
Revision as of 17:16, 21 February 2008
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REFINEMENT OF GLUCOSE ISOMERASE FROM STREPTOMYCES ALBUS AT 1.65 ANGSTROMS WITH DATA FROM AN IMAGING PLATE
Overview
The structure of 'metal-free' glucose isomerase of Streptomyces albus strain number YT ATCC 21132 has been analysed and refined at 1.65 A. The space group is I222, with cell dimensions a = 93.9 (1), b = 99.7 (1) and c = 102.9 (1) A, and there is one monomer of the tetrameric molecule per asymmetric unit. The data were recorded from two crystals of the protein using synchrotron radiation from the EMBL beamline X11 at DESY, Hamburg. Data were recorded with an imaging plate scanner designed and built in the EMBL Hamburg outstation. The total data-collection time was less than 12 h and the processing of all data took less than 2 days. The coordinates of the Arthrobacter glucose isomerase refined at a resolution of 2.5 A were used as a starting model. The structure of the protein and of 445 associated water molecules in the asymmetric unit were refined by restrained least-squares minimization using all data between 8 and 1.65 A to a final R factor of 14.1%.
About this Structure
6XIA is a Single protein structure of sequence from Streptomyces albus. Active as Xylose isomerase, with EC number 5.3.1.5 Full crystallographic information is available from OCA.
Reference
Refinement of glucose isomerase from Streptomyces albus at 1.65 A with data from an imaging plate., Dauter Z, Terry H, Witzel H, Wilson KS, Acta Crystallogr B. 1990 Dec 1;46 ( Pt 6):833-41. PMID:2085424
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