7cat
From Proteopedia
(New page: 200px<br /><applet load="7cat" size="350" color="white" frame="true" align="right" spinBox="true" caption="7cat, resolution 2.5Å" /> '''THE NADPH BINDING SIT...) |
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==Overview== | ==Overview== | ||
| - | Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP | + | Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Catalase]] | [[Category: Catalase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: /III, T | + | [[Category: /III, T J.Reid.]] |
[[Category: Fita, I.]] | [[Category: Fita, I.]] | ||
| - | [[Category: Murthy, M | + | [[Category: Murthy, M R.N.]] |
| - | [[Category: Rossmann, M | + | [[Category: Rossmann, M G.]] |
[[Category: Sicignano, A.]] | [[Category: Sicignano, A.]] | ||
[[Category: Tanaka, N.]] | [[Category: Tanaka, N.]] | ||
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[[Category: oxidoreductase (h2o2 acceptor)]] | [[Category: oxidoreductase (h2o2 acceptor)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:16:57 2008'' |
Revision as of 17:17, 21 February 2008
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THE NADPH BINDING SITE ON BEEF LIVER CATALASE
Overview
Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery.
About this Structure
7CAT is a Single protein structure of sequence from [1] with and as ligands. Active as Catalase, with EC number 1.11.1.6 Full crystallographic information is available from OCA.
Reference
The NADPH binding site on beef liver catalase., Fita I, Rossmann MG, Proc Natl Acad Sci U S A. 1985 Mar;82(6):1604-8. PMID:3856839
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