7ins
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The crystal structure of NPH-insulin, pig insulin cocrystallized with | + | The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods. The final crystallographic R factor for all reflections between 2 and 10 A is 19.4%. The insulin molecules are arranged as hexamers with two tetrahedrally coordinated Zn atoms in the central channel and one m-cresol bound to each monomer near His B5. One protamine binding site has been unequivocally identified near a dimer-dimer interface, although most of the polypeptide is crystallographically disordered. The conformation of the insulin moiety and the structural differences between the three unique monomers have been analysed. The zinc and m-cresol environments are described and the nature of the protamine binding site is outlined. |
==About this Structure== | ==About this Structure== | ||
| - | 7INS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=UNK:'>UNK</scene> and <scene name='pdbligand=CRS:'>CRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MCR:M-Cresol Binding Site. Oh Binds On Each Monomer To Oa6 A ...'>MCR</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7INS OCA]. | + | 7INS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=UNK:'>UNK</scene> and <scene name='pdbligand=CRS:'>CRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MCR:M-Cresol+Binding+Site.+Oh+Binds+On+Each+Monomer+To+Oa6+A+...'>MCR</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7INS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dodson, E.]] | [[Category: Dodson, E.]] | ||
[[Category: Dodson, G.]] | [[Category: Dodson, G.]] | ||
| - | [[Category: Hansen, F | + | [[Category: Hansen, F B.]] |
[[Category: Korber, F.]] | [[Category: Korber, F.]] | ||
[[Category: CRS]] | [[Category: CRS]] | ||
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[[Category: hormone]] | [[Category: hormone]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:17:21 2008'' |
Revision as of 17:17, 21 February 2008
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STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z
Overview
The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods. The final crystallographic R factor for all reflections between 2 and 10 A is 19.4%. The insulin molecules are arranged as hexamers with two tetrahedrally coordinated Zn atoms in the central channel and one m-cresol bound to each monomer near His B5. One protamine binding site has been unequivocally identified near a dimer-dimer interface, although most of the polypeptide is crystallographically disordered. The conformation of the insulin moiety and the structural differences between the three unique monomers have been analysed. The zinc and m-cresol environments are described and the nature of the protamine binding site is outlined.
About this Structure
7INS is a Protein complex structure of sequences from Sus scrofa with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of porcine insulin cocrystallized with clupeine Z., Balschmidt P, Hansen FB, Dodson EJ, Dodson GG, Korber F, Acta Crystallogr B. 1991 Dec 1;47 ( Pt 6):975-86. PMID:1772633
Page seeded by OCA on Thu Feb 21 19:17:21 2008
Categories: Protein complex | Sus scrofa | Balschmidt, P. | Dodson, E. | Dodson, G. | Hansen, F B. | Korber, F. | CRS | UNK | ZN | Hormone
