7msf
From Proteopedia
(New page: 200px<br /><applet load="7msf" size="450" color="white" frame="true" align="right" spinBox="true" caption="7msf, resolution 2.8Å" /> '''MS2 PROTEIN CAPSID/RN...) |
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| - | [[Image:7msf.gif|left|200px]]<br /><applet load="7msf" size=" | + | [[Image:7msf.gif|left|200px]]<br /><applet load="7msf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="7msf, resolution 2.8Å" /> | caption="7msf, resolution 2.8Å" /> | ||
'''MS2 PROTEIN CAPSID/RNA COMPLEX'''<br /> | '''MS2 PROTEIN CAPSID/RNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the crystal structures, at 2.8 A resolution, of two | + | We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs. |
==About this Structure== | ==About this Structure== | ||
| - | 7MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http:// | + | 7MSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MSF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacterio phage ms2]] | [[Category: Enterobacterio phage ms2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Adams, C | + | [[Category: Adams, C J.]] |
| - | [[Category: Convery, M | + | [[Category: Convery, M A.]] |
| - | [[Category: Ellington, A | + | [[Category: Ellington, A D.]] |
[[Category: Hirao, I.]] | [[Category: Hirao, I.]] | ||
| - | [[Category: Peabody, D | + | [[Category: Peabody, D S.]] |
| - | [[Category: Phillips, S | + | [[Category: Phillips, S E.V.]] |
[[Category: Rowsell, S.]] | [[Category: Rowsell, S.]] | ||
| - | [[Category: Stockley, P | + | [[Category: Stockley, P G.]] |
| - | [[Category: Stonehouse, N | + | [[Category: Stonehouse, N J.]] |
[[Category: bacteriophage ms2]] | [[Category: bacteriophage ms2]] | ||
[[Category: ccapsid protein ms2/rna aptamer complex]] | [[Category: ccapsid protein ms2/rna aptamer complex]] | ||
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[[Category: rna-protein complex]] | [[Category: rna-protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:17:24 2008'' |
Revision as of 17:17, 21 February 2008
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MS2 PROTEIN CAPSID/RNA COMPLEX
Overview
We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.
About this Structure
7MSF is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
Reference
Crystal structures of a series of RNA aptamers complexed to the same protein target., Rowsell S, Stonehouse NJ, Convery MA, Adams CJ, Ellington AD, Hirao I, Peabody DS, Stockley PG, Phillips SE, Nat Struct Biol. 1998 Nov;5(11):970-5. PMID:9808042
Page seeded by OCA on Thu Feb 21 19:17:24 2008
Categories: Enterobacterio phage ms2 | Single protein | Adams, C J. | Convery, M A. | Ellington, A D. | Hirao, I. | Peabody, D S. | Phillips, S E.V. | Rowsell, S. | Stockley, P G. | Stonehouse, N J. | Bacteriophage ms2 | Ccapsid protein ms2/rna aptamer complex | Icosahedral virus | Rna stem loop | Rna-protein complex
