8cgt
From Proteopedia
(New page: 200px<br /><applet load="8cgt" size="450" color="white" frame="true" align="right" spinBox="true" caption="8cgt, resolution 2.4Å" /> '''STRUCTURE OF CYCLODEX...) |
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| - | [[Image:8cgt.gif|left|200px]]<br /><applet load="8cgt" size=" | + | [[Image:8cgt.gif|left|200px]]<br /><applet load="8cgt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="8cgt, resolution 2.4Å" /> | caption="8cgt, resolution 2.4Å" /> | ||
'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH A THIO-MALTOHEXAOSE'''<br /> | '''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH A THIO-MALTOHEXAOSE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic | + | Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds. |
==About this Structure== | ==About this Structure== | ||
| - | 8CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with CA and TM6 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http:// | + | 8CGT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=TM6:'>TM6</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CGT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cyclomaltodextrin glucanotransferase]] | [[Category: Cyclomaltodextrin glucanotransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Schmidt, A | + | [[Category: Schmidt, A K.]] |
| - | [[Category: Schulz, G | + | [[Category: Schulz, G E.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: TM6]] | [[Category: TM6]] | ||
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[[Category: starch degradation]] | [[Category: starch degradation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:17:50 2008'' |
Revision as of 17:17, 21 February 2008
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STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH A THIO-MALTOHEXAOSE
Overview
Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have been performed with several substrates and analogues. The observed binding modes differ in detail, but agree in general with data on homologous enzymes. Based on these binding studies, two mutations were designed that changed the production spectrum from the predominant product beta-cyclodextrin of the wild-type enzyme towards gamma-cyclodextrin, which is of practical interest because it is rare and can encapsulate larger nonpolar compounds.
About this Structure
8CGT is a Single protein structure of sequence from Bacillus circulans with and as ligands. Active as Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 Full crystallographic information is available from OCA.
Reference
Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the gamma-cyclodextrin production., Parsiegla G, Schmidt AK, Schulz GE, Eur J Biochem. 1998 Aug 1;255(3):710-7. PMID:9738912
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