8nse
From Proteopedia
(New page: 200px<br /><applet load="8nse" size="450" color="white" frame="true" align="right" spinBox="true" caption="8nse, resolution 2.25Å" /> '''BOVINE ENDOTHELIAL N...) |
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| - | [[Image:8nse.gif|left|200px]]<br /><applet load="8nse" size=" | + | [[Image:8nse.gif|left|200px]]<br /><applet load="8nse" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="8nse, resolution 2.25Å" /> | caption="8nse, resolution 2.25Å" /> | ||
'''BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE, NNA COMPLEX'''<br /> | '''BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE, NNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Nitric oxide is generated under normal and pathophysiological conditions | + | Nitric oxide is generated under normal and pathophysiological conditions by three distinct isoforms of nitric oxide synthase (NOS). A small-molecule inhibitor of NOS (3-Br-7-nitroindazole, 7-NIBr) is profoundly neuroprotective in mouse models of stroke and Parkinson's disease. We report the crystal structure of the catalytic heme domain of endothelial NOS complexed with 7-NIBr at 1.65 A resolution. Critical to the binding of 7-NIBr at the substrate site is the adoption by eNOS of an altered conformation, in which a key glutamate residue swings out toward one of the heme propionate groups. Perturbation of the heme propionate ensues and eliminates the cofactor tetrahydrobiopterin-heme interaction. We also present three crystal structures that reveal how alterations at the substrate site facilitate 7-NIBr and structurally dissimilar ligands to occupy the cofactor site. |
==About this Structure== | ==About this Structure== | ||
| - | 8NSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with ZN, HEM, H4B, NRG, CAD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http:// | + | 8NSE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=H4B:'>H4B</scene>, <scene name='pdbligand=NRG:'>NRG</scene>, <scene name='pdbligand=CAD:'>CAD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8NSE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Li, H.]] | [[Category: Li, H.]] | ||
[[Category: Martasek, P.]] | [[Category: Martasek, P.]] | ||
| - | [[Category: Masters, B | + | [[Category: Masters, B S.S.]] |
| - | [[Category: Poulos, T | + | [[Category: Poulos, T L.]] |
| - | [[Category: Raman, C | + | [[Category: Raman, C S.]] |
[[Category: CAD]] | [[Category: CAD]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: tetrahydrobiopterin]] | [[Category: tetrahydrobiopterin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:18:13 2008'' |
Revision as of 17:18, 21 February 2008
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BOVINE ENDOTHELIAL NITRIC OXIDE SYNTHASE, NNA COMPLEX
Overview
Nitric oxide is generated under normal and pathophysiological conditions by three distinct isoforms of nitric oxide synthase (NOS). A small-molecule inhibitor of NOS (3-Br-7-nitroindazole, 7-NIBr) is profoundly neuroprotective in mouse models of stroke and Parkinson's disease. We report the crystal structure of the catalytic heme domain of endothelial NOS complexed with 7-NIBr at 1.65 A resolution. Critical to the binding of 7-NIBr at the substrate site is the adoption by eNOS of an altered conformation, in which a key glutamate residue swings out toward one of the heme propionate groups. Perturbation of the heme propionate ensues and eliminates the cofactor tetrahydrobiopterin-heme interaction. We also present three crystal structures that reveal how alterations at the substrate site facilitate 7-NIBr and structurally dissimilar ligands to occupy the cofactor site.
About this Structure
8NSE is a Single protein structure of sequence from Bos taurus with , , , , and as ligands. Active as Nitric-oxide synthase, with EC number 1.14.13.39 Full crystallographic information is available from OCA.
Reference
Crystal structure of nitric oxide synthase bound to nitro indazole reveals a novel inactivation mechanism., Raman CS, Li H, Martasek P, Southan G, Masters BS, Poulos TL, Biochemistry. 2001 Nov 13;40(45):13448-55. PMID:11695891
Page seeded by OCA on Thu Feb 21 19:18:13 2008
Categories: Bos taurus | Nitric-oxide synthase | Single protein | Li, H. | Martasek, P. | Masters, B S.S. | Poulos, T L. | Raman, C S. | CAD | GOL | H4B | HEM | NRG | ZN | Heme protein | Nitric oxide synthase | Tetrahydrobiopterin
