9ant
From Proteopedia
(New page: 200px<br /><applet load="9ant" size="450" color="white" frame="true" align="right" spinBox="true" caption="9ant, resolution 2.40Å" /> '''ANTENNAPEDIA HOMEODO...) |
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| - | [[Image:9ant.gif|left|200px]]<br /><applet load="9ant" size=" | + | [[Image:9ant.gif|left|200px]]<br /><applet load="9ant" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="9ant, resolution 2.40Å" /> | caption="9ant, resolution 2.40Å" /> | ||
'''ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX'''<br /> | '''ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Homeodomains are one of the key families of eukaryotic DNA-binding motifs | + | Homeodomains are one of the key families of eukaryotic DNA-binding motifs and provide an important model system for studying protein-DNA interactions. We have crystallized the Antennapedia homeodomain-DNA complex and solved this structure at 2.4 A resolution. NMR and molecular dynamics studies had implied that this homeodomain achieves specificity through an ensemble of rapidly fluctuating DNA contacts. The crystal structure is in agreement with the underlying NMR data, but our structure reveals a well-defined set of contacts and also reveals the locations and roles of water molecules at the protein-DNA interface. The synthesis of X-ray and NMR studies provides a unified, general model for homeodomain-DNA interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 9ANT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 9ANT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ANT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Fraenkel, E.]] | [[Category: Fraenkel, E.]] | ||
| - | [[Category: Pabo, C | + | [[Category: Pabo, C O.]] |
[[Category: NI]] | [[Category: NI]] | ||
[[Category: complex (homeodomain/dna)]] | [[Category: complex (homeodomain/dna)]] | ||
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[[Category: homeodomain]] | [[Category: homeodomain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:18:26 2008'' |
Revision as of 17:18, 21 February 2008
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ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX
Overview
Homeodomains are one of the key families of eukaryotic DNA-binding motifs and provide an important model system for studying protein-DNA interactions. We have crystallized the Antennapedia homeodomain-DNA complex and solved this structure at 2.4 A resolution. NMR and molecular dynamics studies had implied that this homeodomain achieves specificity through an ensemble of rapidly fluctuating DNA contacts. The crystal structure is in agreement with the underlying NMR data, but our structure reveals a well-defined set of contacts and also reveals the locations and roles of water molecules at the protein-DNA interface. The synthesis of X-ray and NMR studies provides a unified, general model for homeodomain-DNA interactions.
About this Structure
9ANT is a Single protein structure of sequence from Drosophila melanogaster with as ligand. Full crystallographic information is available from OCA.
Reference
Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex., Fraenkel E, Pabo CO, Nat Struct Biol. 1998 Aug;5(8):692-7. PMID:9699632
Page seeded by OCA on Thu Feb 21 19:18:26 2008
