9gaf
From Proteopedia
(New page: 200px<br /><applet load="9gaf" size="450" color="white" frame="true" align="right" spinBox="true" caption="9gaf, resolution 1.9Å" /> '''PRECURSOR OF THE W11F...) |
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| - | [[Image:9gaf.gif|left|200px]]<br /><applet load="9gaf" size=" | + | [[Image:9gaf.gif|left|200px]]<br /><applet load="9gaf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="9gaf, resolution 1.9Å" /> | caption="9gaf, resolution 1.9Å" /> | ||
'''PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''<br /> | '''PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A variety of proteins, including glycosylasparaginase, have recently been | + | A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --> O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --> O or N --> S acyl shift that is used by various groups of intramolecular autoprocessing proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 9GAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica] with GLY as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] Full crystallographic information is available from [http:// | + | 9GAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica] with <scene name='pdbligand=GLY:'>GLY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]] | [[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Guo, H | + | [[Category: Guo, H C.]] |
[[Category: Xu, Q.]] | [[Category: Xu, Q.]] | ||
[[Category: GLY]] | [[Category: GLY]] | ||
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[[Category: precursor]] | [[Category: precursor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:18:30 2008'' |
Revision as of 17:18, 21 February 2008
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PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM
Overview
A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --> O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --> O or N --> S acyl shift that is used by various groups of intramolecular autoprocessing proteins.
About this Structure
9GAF is a Single protein structure of sequence from Elizabethkingia meningoseptica with as ligand. Active as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase, with EC number 3.5.1.26 Full crystallographic information is available from OCA.
Reference
Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61. PMID:10490104
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