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2qfb
From Proteopedia
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cui, S.]] | [[Category: Cui, S.]] | ||
| + | [[Category: Hopfner, K P.]] | ||
| + | [[Category: Lammens, A.]] | ||
| + | [[Category: Lammens, K.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:47:59 2008'' |
Revision as of 05:48, 27 February 2008
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Crystal structure of the regulatory domain of human RIG-I with bound Zn
Overview
The ATPase RIG-I senses viral RNAs that contain 5'-triphosphates in the cytoplasm. It initiates a signaling cascade that activates innate immune response by interferon and cytokine production, providing essential antiviral protection for the host. The mode of RNA 5'-triphosphate sensing by RIG-I remains elusive. We show that the C-terminal regulatory domain RD of RIG-I binds viral RNA in a 5'-triphosphate-dependent manner and activates the RIG-I ATPase by RNA-dependent dimerization. The crystal structure of RD reveals a zinc-binding domain that is structurally related to GDP/GTP exchange factors of Rab-like GTPases. The zinc coordination site is essential for RIG-I signaling and is also conserved in MDA5 and LGP2, suggesting related RD domains in all three enzymes. Structure-guided mutagenesis identifies a positively charged groove as likely 5'-triphosphate-binding site of RIG-I. This groove is distinct in MDA5 and LGP2, raising the possibility that RD confers ligand specificity.
About this Structure
2QFB is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Sites: , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
The C-Terminal Regulatory Domain Is the RNA 5'-Triphosphate Sensor of RIG-I., Cui S, Eisenacher K, Kirchhofer A, Brzozka K, Lammens A, Lammens K, Fujita T, Conzelmann KK, Krug A, Hopfner KP, Mol Cell. 2008 Feb 1;29(2):169-179. PMID:18243112
Page seeded by OCA on Wed Feb 27 07:47:59 2008
Categories: Homo sapiens | Single protein | Cui, S. | Hopfner, K P. | Lammens, A. | Lammens, K. | ZN | Alternative splicing | Antiviral defense | Atp-binding | Helicase | Hydrolase | Immune response | Innate immunity | Interferon induction | Nucleotide-binding | Polymorphism | Rna-binding | Ubl conjugation | Zinc finger
