Proteinase
From Proteopedia
(Difference between revisions)
(New page: <StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code 2duj)' scene=''> '''Proteinase''' (PRO) are enz...) |
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<StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code [[2duj]])' scene=''> | <StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code [[2duj]])' scene=''> | ||
| - | '''Proteinase''' (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details on PRO B see [[Streptomyces griseus proteinase B]]. PRO A is a carboxylproteinase. | + | '''Proteinase''' (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details on PRO B see [[Streptomyces griseus proteinase B]]. PRO A is a carboxylproteinase. PRO K is a serine protease which cleaves proteins preferentially after hydrophobic residues. Calcium ions contribute to the stability of the enzyme. PRO is active over a wide pH range and is used in molecular biology to inactivate nucleases from preparations of DNA or RNA. PRO is used in the partial proteolysis of lactoferrin into its N- and C-lobe. The two lobes of lactoferrin have different antimicrobial and antifungal properties. PRO K can digest hair (keratin). |
==3D structures of proteinase== | ==3D structures of proteinase== | ||
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'''PRO A''' | '''PRO A''' | ||
| - | 2sga – SgPRO – ''Streptomyces griseus'' | + | [[2sga]] – SgPRO – ''Streptomyces griseus''<br /> |
| - | 2jxr, 1fmu, 1fmx – yPRO - yeast | + | [[2jxr]], [[1fmu]], [[1fmx]] – yPRO - yeast<br /> |
| - | 1sgc - SgPRO + chymostatin A | + | [[1sgc]] - SgPRO + chymostatin A<br /> |
| - | 3sga, 4sga, 5sga - SgPRO + polypeptide inhibitor | + | [[3sga]], [[4sga]], [[5sga]] - SgPRO + polypeptide inhibitor<br /> |
| - | 1dp5, 1dpj, 1g0v - yPRO + polypeptide inhibitor IA3 | + | [[1dp5]], [[1dpj]], [[1g0v]] - yPRO + polypeptide inhibitor IA3<br /> |
| - | 1fq5 - yPRO + inhibitor | + | [[1fq5]] - yPRO + inhibitor |
'''PRO B''' | '''PRO B''' | ||
| - | + | [[sgb]] – SgPRO + turkey ovomucoid inhibitor<br /> | |
| - | 1sgp, 1sgq, 1sgr, 1cso, 1ct0, 1ct2, 1ct4, 1ds2, 2sgp, 2nu3, 2nu4 – SgPRO + turkey ovomucoid inhibitor (mutant) | + | [[1sgp]], [[1sgq]], [[1sgr]], [[1cso]], [[1ct0]], [[1ct2]], [[1ct4]], [[1ds2]], [[2sgp]], [[2nu3]], [[2nu4]] – SgPRO + turkey ovomucoid inhibitor (mutant)<br /> |
| - | 4sgb - SgPRO + potato inhibitor | + | [[4sgb]] - SgPRO + potato inhibitor |
'''PRO K''' | '''PRO K''' | ||
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[[1oyo]] – EaPRO + Ca + melanin monomer <br /> | [[1oyo]] – EaPRO + Ca + melanin monomer <br /> | ||
[[3dyb]] – EaPRO + Ca + digalacturonic acid <br /> | [[3dyb]] – EaPRO + Ca + digalacturonic acid <br /> | ||
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'''PRO 3C''' | '''PRO 3C''' | ||
| - | 1qa7 – PRO – Hepatitis virus | + | [[1qa7]] – PRO – Hepatitis virus<br /> |
| - | 2vb0 - PRO – Coxsakievirus | + | [[2vb0]] - PRO – Coxsakievirus |
'''H2-PRO''' | '''H2-PRO''' | ||
| - | 1wni – PRO – ''Trimeresurus flavoviridis'' | + | [[1wni]] – PRO – ''Trimeresurus flavoviridis'' |
'''Aspartic PRO''' | '''Aspartic PRO''' | ||
| - | 2asi – PRO – ''Rhizomucor miehei'' | + | [[2asi]] – PRO – ''Rhizomucor miehei''<br /> |
| - | 1zap – CaPRO – ''Candida albicans'' | + | [[1zap]] – CaPRO – ''Candida albicans''<br /> |
| - | 1izd - AoPRO – ''Aspergillus oryzae'' | + | [[1izd]] - AoPRO – ''Aspergillus oryzae''<br /> |
| - | 1eag – CaPRO + inhibitor | + | [[1eag]] – CaPRO + inhibitor <br /> |
| - | 1fq4 - yPRO + inhibitor | + | [[1fq4]] - yPRO + inhibitor<br /> |
| - | 1j71 - PRO + polypeptide inhibitor – ''Candida tropicalis'' | + | [[1j71]] - PRO + polypeptide inhibitor – ''Candida tropicalis''<br /> |
| - | 1ize - AoPRO + polypeptide-statine inhibitor | + | [[1ize]] - AoPRO + polypeptide-statine inhibitor<br /> |
| - | 1wkr - PRO + polypeptide-statine inhibitor – ''Irpex lacteus'' | + | [[1wkr]] - PRO + polypeptide-statine inhibitor – ''Irpex lacteus'' |
'''Cysteine PRO''' | '''Cysteine PRO''' | ||
| - | 2hrv – PRO 2A – human rhinovirus | + | [[2hrv]] – PRO 2A – human rhinovirus |
'''Serine PRO''' | '''Serine PRO''' | ||
| - | 1s2n, 1sh7 – PRO – Vibrio | + | [[1s2n]], [[1sh7]] – PRO – ''Vibrio''<br /> |
| - | 3s9a, 3s9b – RvPRO – Siamese Russell’s viper | + | [[3s9a]], [[3s9b]] – RvPRO – Siamese Russell’s viper<br /> |
| - | 3s9c, 3sbk – RvPRO + human factor V polypeptide | + | [[3s9c]], [[3sbk]] – RvPRO + human factor V polypeptide<br /> |
| - | 1ga1, 1ga4, 1ga6, 1nlu – PsPRO + iodotyrostatin fragment – ''Pseudomonas'' | + | [[1ga1]], [[1ga4]], [[1ga6]], [[1nlu]] – PsPRO + iodotyrostatin fragment – ''Pseudomonas''<br /> |
| - | 1kdv, 1kdy, 1kdz, 1ke1, 1ke2 - PsPRO + polypeptide inhibitor | + | [[1kdv]], [[1kdy]], [[1kdz]], [[1ke1]], [[1ke2]] - PsPRO + polypeptide inhibitor |
Revision as of 08:43, 16 April 2014
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References
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Joel L. Sussman, Alexander Berchansky, Karsten Theis
