1kjy
From Proteopedia
m (Protected "1kjy" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1kjy.png|left|200px]] | ||
| - | |||
| - | <!-- | ||
| - | The line below this paragraph, containing "STRUCTURE_1kjy", creates the "Structure Box" on the page. | ||
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet) | ||
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| - | or leave the SCENE parameter empty for the default display. | ||
| - | --> | ||
{{STRUCTURE_1kjy| PDB=1kjy | SCENE= }} | {{STRUCTURE_1kjy| PDB=1kjy | SCENE= }} | ||
| - | |||
===Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14=== | ===Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14=== | ||
| + | {{ABSTRACT_PUBMED_11976690}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref> [[http://www.uniprot.org/uniprot/RGS14_RAT RGS14_RAT]] Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.<ref>PMID:11387333</ref> <ref>PMID:15337739</ref> <ref>PMID:19319189</ref> <ref>PMID:19574389</ref> <ref>PMID:19878719</ref> <ref>PMID:21255223</ref> <ref>PMID:21158412</ref> | |
| - | The | + | |
| - | + | ||
| - | --> | + | |
| - | + | ||
==About this Structure== | ==About this Structure== | ||
[[1kjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJY OCA]. | [[1kjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJY OCA]. | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Guanine nucleotide-binding protein|Guanine nucleotide-binding protein]] | ||
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:011976690</ref><references group="xtra"/> | + | <ref group="xtra">PMID:011976690</ref><references group="xtra"/><references/> |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Betts, L.]] | [[Category: Betts, L.]] | ||
Revision as of 10:57, 16 April 2014
Contents |
Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14
Template:ABSTRACT PUBMED 11976690
Function
[GNAI1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.[1] [2] [RGS14_RAT] Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division; required for completion of the first mitotic division of the embryo. Involved in visual memory processing capacity; when overexpressed in the V2 secondary visual cortex area. Involved in hippocampal-based learning and memory; acts as a suppressor of synaptic plasticity in CA2 neurons. Required for the nerve growth factor (NGF)-mediated neurite outgrowth. Involved in stress resistance.[3] [4] [5] [6] [7] [8] [9]
About this Structure
1kjy is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
See Also
Reference
- Kimple RJ, Kimple ME, Betts L, Sondek J, Siderovski DP. Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits. Nature. 2002 Apr 25;416(6883):878-81. PMID:11976690 doi:http://dx.doi.org/10.1038/416878a
- ↑ Cho H, Kehrl JH. Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division. J Cell Biol. 2007 Jul 16;178(2):245-55. PMID:17635935 doi:10.1083/jcb.200604114
- ↑ Johnston CA, Siderovski DP. Structural basis for nucleotide exchange on G alpha i subunits and receptor coupling specificity. Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):2001-6. Epub 2007 Jan 30. PMID:17264214
- ↑ Kimple RJ, De Vries L, Tronchere H, Behe CI, Morris RA, Gist Farquhar M, Siderovski DP. RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity. J Biol Chem. 2001 Aug 3;276(31):29275-81. Epub 2001 May 31. PMID:11387333 doi:http://dx.doi.org/10.1074/jbc.M103208200
- ↑ Mittal V, Linder ME. The RGS14 GoLoco domain discriminates among Galphai isoforms. J Biol Chem. 2004 Nov 5;279(45):46772-8. Epub 2004 Aug 26. PMID:15337739 doi:http://dx.doi.org/10.1074/jbc.M407409200
- ↑ Willard FS, Willard MD, Kimple AJ, Soundararajan M, Oestreich EA, Li X, Sowa NA, Kimple RJ, Doyle DA, Der CJ, Zylka MJ, Snider WD, Siderovski DP. Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector. PLoS One. 2009;4(3):e4884. doi: 10.1371/journal.pone.0004884. Epub 2009 Mar 25. PMID:19319189 doi:http://dx.doi.org/10.1371/journal.pone.0004884
- ↑ Lopez-Aranda MF, Lopez-Tellez JF, Navarro-Lobato I, Masmudi-Martin M, Gutierrez A, Khan ZU. Role of layer 6 of V2 visual cortex in object-recognition memory. Science. 2009 Jul 3;325(5936):87-9. doi: 10.1126/science.1170869. PMID:19574389 doi:http://dx.doi.org/10.1126/science.1170869
- ↑ Shu FJ, Ramineni S, Hepler JR. RGS14 is a multifunctional scaffold that integrates G protein and Ras/Raf MAPkinase signalling pathways. Cell Signal. 2010 Mar;22(3):366-76. doi: 10.1016/j.cellsig.2009.10.005. Epub . PMID:19878719 doi:http://dx.doi.org/10.1016/j.cellsig.2009.10.005
- ↑ Lin YR, Kim K, Yang Y, Ivessa A, Sadoshima J, Park Y. Regulation of longevity by regulator of G-protein signaling protein, Loco. Aging Cell. 2011 Jun;10(3):438-47. doi: 10.1111/j.1474-9726.2011.00678.x. Epub, 2011 Mar 22. PMID:21255223 doi:http://dx.doi.org/10.1111/j.1474-9726.2011.00678.x
- ↑ Vellano CP, Shu FJ, Ramineni S, Yates CK, Tall GG, Hepler JR. Activation of the regulator of G protein signaling 14-Galphai1-GDP signaling complex is regulated by resistance to inhibitors of cholinesterase-8A. Biochemistry. 2011 Feb 8;50(5):752-62. doi: 10.1021/bi101910n. Epub 2011 Jan 11. PMID:21158412 doi:http://dx.doi.org/10.1021/bi101910n
