2ves
From Proteopedia
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caption="2ves, resolution 1.90Å" /> | caption="2ves, resolution 1.90Å" /> | ||
'''CRYSTAL STRUCTURE OF LPXC FROM PSEUDOMONAS AERUGINOSA COMPLEXED WITH THE POTENT BB-78485 INHIBITOR'''<br /> | '''CRYSTAL STRUCTURE OF LPXC FROM PSEUDOMONAS AERUGINOSA COMPLEXED WITH THE POTENT BB-78485 INHIBITOR'''<br /> | ||
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| + | ==Overview== | ||
| + | The cell wall in Gram-negative bacteria is surrounded by an outer membrane comprised of charged lipopolysaccharide (LPS) molecules that prevent entry of hydrophobic agents into the cell and protect the bacterium from many antibiotics. The hydrophobic anchor of LPS is lipid A, the biosynthesis of which is essential for bacterial growth and viability. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) is an essential zinc-dependant enzyme that catalyzes the conversion of UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)glucosamine and acetate in the biosynthesis of lipid A, and for this reason, LpxC is an attractive target for antibacterial drug discovery. Here we disclose a 1.9 A resolution crystal structure of LpxC from Pseudomonas aeruginosa (paLpxC) in a complex with the potent BB-78485 inhibitor. To our knowledge, this is the first crystal structure of LpxC with a small-molecule inhibitor that shows antibacterial activity against a wide range of Gram-negative pathogens. Accordingly, this structure can provide important information for lead optimization and rational design of the effective small-molecule LpxC inhibitors for successful treatment of Gram-negative infections. | ||
==About this Structure== | ==About this Structure== | ||
2VES is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GVR:'>GVR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:Gvr+Binding+Site+For+Chain+A'>AC4</scene>, <scene name='pdbsite=AC5:Gvr+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Gvr+Binding+Site+For+Chain+C'>AC6</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Chain+B'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Chain+C'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Chain+C'>BC1</scene>, <scene name='pdbsite=BC2:So4+Binding+Site+For+Chain+B'>BC2</scene> and <scene name='pdbsite=BC3:So4+Binding+Site+For+Chain+C'>BC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VES OCA]. | 2VES is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GVR:'>GVR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:Gvr+Binding+Site+For+Chain+A'>AC4</scene>, <scene name='pdbsite=AC5:Gvr+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Gvr+Binding+Site+For+Chain+C'>AC6</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Chain+A'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Chain+B'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Chain+C'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Chain+C'>BC1</scene>, <scene name='pdbsite=BC2:So4+Binding+Site+For+Chain+B'>BC2</scene> and <scene name='pdbsite=BC3:So4+Binding+Site+For+Chain+C'>BC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VES OCA]. | ||
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| + | ==Reference== | ||
| + | Crystal structure of LpxC from Pseudomonas aeruginosa complexed with the potent BB-78485 inhibitor., Mochalkin I, Knafels JD, Lightle S, Protein Sci. 2008 Mar;17(3):450-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18287278 18287278] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: | + | [[Category: antibiotic]] |
[[Category: bb-78485]] | [[Category: bb-78485]] | ||
[[Category: deacetylase]] | [[Category: deacetylase]] | ||
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[[Category: metalloprotease]] | [[Category: metalloprotease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 5 13:25:52 2008'' |
Revision as of 11:25, 5 March 2008
|
CRYSTAL STRUCTURE OF LPXC FROM PSEUDOMONAS AERUGINOSA COMPLEXED WITH THE POTENT BB-78485 INHIBITOR
Overview
The cell wall in Gram-negative bacteria is surrounded by an outer membrane comprised of charged lipopolysaccharide (LPS) molecules that prevent entry of hydrophobic agents into the cell and protect the bacterium from many antibiotics. The hydrophobic anchor of LPS is lipid A, the biosynthesis of which is essential for bacterial growth and viability. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) is an essential zinc-dependant enzyme that catalyzes the conversion of UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)glucosamine and acetate in the biosynthesis of lipid A, and for this reason, LpxC is an attractive target for antibacterial drug discovery. Here we disclose a 1.9 A resolution crystal structure of LpxC from Pseudomonas aeruginosa (paLpxC) in a complex with the potent BB-78485 inhibitor. To our knowledge, this is the first crystal structure of LpxC with a small-molecule inhibitor that shows antibacterial activity against a wide range of Gram-negative pathogens. Accordingly, this structure can provide important information for lead optimization and rational design of the effective small-molecule LpxC inhibitors for successful treatment of Gram-negative infections.
About this Structure
2VES is a Single protein structure of sequence from Pseudomonas aeruginosa with , and as ligands. Known structural/functional Sites: , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of LpxC from Pseudomonas aeruginosa complexed with the potent BB-78485 inhibitor., Mochalkin I, Knafels JD, Lightle S, Protein Sci. 2008 Mar;17(3):450-7. PMID:18287278
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