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4ou0

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'''Unreleased structure'''
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==Crystal Structure of RPA32C==
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<StructureSection load='4ou0' size='340' side='right' caption='[[4ou0]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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[[4ou0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU0 OCA]. <br>
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<b>Related:</b> [[1dpu|1dpu]]<br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A-like1 (SMARCAL1) is a recently identified DNA damage response protein involved in remodeling stalled replication forks. The eukaryotic single-strand (ss) DNA-binding protein Replication Protein A (RPA) recruits SMARCAL1 to stalled forks in vivo and facilitates regression of forks containing leading strand gaps. Both activities are mediated by a direct interaction between an RPA-binding motif (RBM) at the N-terminus of SMARCAL1 and the C-terminal winged-helix domain of the RPA 32-kDa subunit (RPA32C). Here we report a biophysical and structural characterization of the SMARCAL1-RPA interaction. Isothermal titration calorimetry and CD spectroscopy revealed that RPA32C binds the SMARCAL1-RBM with a Kd of 3 muM and induces a disorder-to-helix transition. The crystal structure of RPA32C was refined to 1.4 A resolution and the SMARCAL1-RBM binding site was mapped on the structure on the basis of NMR chemical shift perturbations. Conservation of the interaction surface to other RBM-containing proteins enabled construction of a model for the RPA32C/SMARCAL1-RBM complex. The implications of our results are discussed with respect to the recruitment of SMARCAL1 and other DNA damage response and repair proteins to stalled replication forks.
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The entry 4ou0 is ON HOLD until Paper Publication
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Structural analysis of RPA recruitment of the DNA damage response protein SMARCAL1.,Feldkamp MD, Mason AC, Eichman BF, Chazin WJ Biochemistry. 2014 Apr 15. PMID:24730652<ref>PMID:24730652</ref>
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Authors: Feldkamp, M.D., Mason, A.C., Eichman, B.F, Chazin, W.J.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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Description: Crystal Structure of RPA32C
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Chazin, W J.]]
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[[Category: Eichman, B F]]
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[[Category: Feldkamp, M D.]]
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[[Category: Mason, A C.]]
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[[Category: Dna binding protein]]
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[[Category: Protein-protein interaction]]
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[[Category: S-methyl-thio-cysteine]]
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[[Category: Winged-helix turn helix]]

Revision as of 07:42, 30 April 2014

Crystal Structure of RPA32C

4ou0, resolution 1.40Å

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