This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4psm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
'''Unreleased structure'''
+
==Crystal structure of pfuThermo-DBP-RP1 (crystal form II)==
 +
<StructureSection load='4psm' size='340' side='right' caption='[[4psm]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
 +
== Structural highlights ==
 +
[[4psm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PSM OCA]. <br>
 +
<b>Related:</b> [[4psl|4psl]], [[4psn|4psn]], [[4pso|4pso]]<br>
 +
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
 +
== Publication Abstract from PubMed ==
 +
Instead of a classical single-stranded deoxyribonuleic acid (DNA)-binding protein (SSB), some hyperthermophilic crenarchaea harbor a non-canonical SSB termed ThermoDBP. Two related but poorly characterized groups of proteins, which share the ThermoDBP N-terminal DNA-binding domain, have a broader phylogenetic distribution and co-exist with ThermoDBPs and/or other SSBs. We have investigated the nucleic acid binding properties and crystal structures of representatives of these groups of ThermoDBP-related proteins (ThermoDBP-RPs) 1 and 2. ThermoDBP-RP 1 and 2 oligomerize by different mechanisms and only ThermoDBP-RP2 exhibits strong single-stranded DNA affinity in vitro. A crystal structure of ThermoDBP-RP2 in complex with DNA reveals how the NTD common to ThermoDBPs and ThermoDBP-RPs can contact the nucleic acid in a manner that allows a symmetric homotetrameric protein complex to bind single-stranded DNA molecules asymmetrically. While single-stranded DNA wraps around the surface or binds along channels of previously investigated SSBs, it traverses an internal, intersubunit tunnel system of a ThermoDBP-RP2 tetramer. Our results indicate that some archaea have acquired special SSBs for genome maintenance in particularly challenging environments.
-
The entry 4psm is ON HOLD until Paper Publication
+
Entrapment of DNA in an intersubunit tunnel system of a single-stranded DNA-binding protein.,Ghalei H, Moeller HV, Eppers D, Sohmen D, Wilson DN, Loll B, Wahl MC Nucleic Acids Res. 2014 Apr 17. PMID:24744237<ref>PMID:24744237</ref>
-
Authors: Gahlei, H., von Moeller, H., Eppers, D., Loll, B., Wahl, M.C.
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
== References ==
-
Description: ssDNA binding protein
+
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Eppers, D.]]
 +
[[Category: Gahlei, H.]]
 +
[[Category: Loll, B.]]
 +
[[Category: Moeller, H von.]]
 +
[[Category: Wahl, M C.]]
 +
[[Category: Dna binding protein]]
 +
[[Category: Ssdna binding protein]]

Revision as of 07:51, 30 April 2014

Crystal structure of pfuThermo-DBP-RP1 (crystal form II)

4psm, resolution 2.43Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4psm"
Personal tools