2li8

Structural highlights

2li8 is a 2 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA.
Related: 2cqf
Activity: Glucokinase, with EC number 2.7.1.2

Publication Abstract from PubMed

Lin28 inhibits the biogenesis of let-7 miRNAs through a direct interaction with the terminal loop of pre-let-7. This interaction requires the zinc-knuckle domains of Lin28. We show that the zinc knuckle domains of Lin28 are sufficient to provide binding selectivity for pre-let-7 miRNAs and present the NMR structure of human Lin28 zinc knuckles bound to the short sequence 5'-AGGAGAU-3'. The structure reveals that each zinc knuckle recognizes an AG dinucleotide separated by a single nucleotide spacer. This defines a new 5'-NGNNG-3' consensus motif that explains how Lin28 selectively recognizes pre-let-7 family members. Binding assays in cell lysates and functional assays in cultured cells demonstrate that the interactions observed in the solution structure also occur between the full-length protein and members of the pre-let-7 family. The consensus sequence explains several seemingly disparate previously published observations on the binding properties of Lin28.

Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28.,Loughlin FE, Gebert LF, Towbin H, Brunschweiger A, Hall J, Allain FH Nat Struct Mol Biol. 2011 Dec 11;19(1):84-9. doi: 10.1038/nsmb.2202. PMID:22157959^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

1. ↑ Loughlin FE, Gebert LF, Towbin H, Brunschweiger A, Hall J, Allain FH. Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28. Nat Struct Mol Biol. 2011 Dec 11;19(1):84-9. doi: 10.1038/nsmb.2202. PMID:22157959 doi:10.1038/nsmb.2202