2lex

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[[Image:2lex.jpg|left|200px]]
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==Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA==
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<StructureSection load='2lex' size='340' side='right' caption='[[2lex]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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[[2lex]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEX OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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The WRKY family transcription factors regulate plant-specific reactions that are mostly related to biotic and abiotic stresses. They share the WRKY domain that recognizes a DNA element (TTCAC[C/T]), termed W-box, in target genes. Here we determined the solution structure of the C-terminal WRKY domain of Arabidopsis WRKY4 in complex with the W-box DNA by NMR. A four-stranded beta-sheet enters the major groove of DNA in an atypical mode termed the beta-wedge, where the sheet is nearly perpendicular to the DNA helical axis. Residues in the conserved WRKYGQK motif contact with DNA bases mainly through extensive apolar contacts with thymine methyl groups. The importance of these contacts was verified by substituting the relevant T bases with U and by surface plasmon resonance (SPR) analyses of DNA binding.
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Structural basis for sequence-specific DNA recognition by an Arabidopsis WRKY transcription factor.,Yamasaki K, Kigawa T, Watanabe S, Inoue M, Yamasaki T, Seki M, Shinozaki K, Yokoyama S J Biol Chem. 2012 Jan 4. PMID:22219184<ref>PMID:22219184</ref>
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The line below this paragraph, containing "STRUCTURE_2lex", creates the "Structure Box" on the page.
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{{STRUCTURE_2lex| PDB=2lex | SCENE= }}
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===Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22219184}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 22219184 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22219184}}
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==About this Structure==
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[[2lex]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEX OCA].
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==Reference==
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<ref group="xtra">PMID:022219184</ref><references group="xtra"/>
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[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Inoue, M.]]
[[Category: Inoue, M.]]

Revision as of 08:29, 30 April 2014

Complex of the C-terminal WRKY domain of AtWRKY4 and a W-box DNA

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