2kyq

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[[Image:2kyq.png|left|200px]]
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==1H, 15N, 13C chemical shifts and structure of CKR-brazzein==
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<StructureSection load='2kyq' size='340' side='right' caption='[[2kyq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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[[2kyq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pentadiplandra_brazzeana Pentadiplandra brazzeana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KYQ OCA]. <br>
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<b>Related:</b> [[2kgq|2kgq]], [[1brz|1brz]]<br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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Brazzein, a 54 residue sweet-tasting protein, is thought to participate in a multipoint binding interaction with the sweet taste receptor. Proposed sites for interaction with the receptor include 2 surface loops and the disulfide bond that connects the N- and C-termini. However, the importance of each site is not well understood. To characterize the structural role of the termini in the sweetness of brazzein, the position of the disulfide bond connecting the N- and C-termini was shifted by substituting K3-C4-K5 with C3-K4-R5. The apparent affinity and V(max) of the C3-K4-R5-brazzein (CKR-brazzein) variant were only modestly decreased compared with the wild-type (WT) brazzein. We determined a high-resolution structure of CKR-brazzein by nuclear magnetic resonance spectroscopy (backbone root mean square deviation of 0.39 A). Comparing the structure of CKR-brazzein with that of WT-brazzein revealed that the terminal beta-strands of the variant display extended beta-structure and increased dynamics relative to WT-brazzein. These results support previous mutagenesis studies and further suggest that, whereas interactions involving the termini are necessary for full function of brazzein, the termini do not constitute the primary site of interaction between brazzein and the sweet taste receptor.
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Structural Role of the Terminal Disulfide Bond in the Sweetness of Brazzein.,Dittli SM, Rao H, Tonelli M, Quijada J, Markley JL, Max M, Assadi-Porter F Chem Senses. 2011 Jul 15. PMID:21765060<ref>PMID:21765060</ref>
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The line below this paragraph, containing "STRUCTURE_2kyq", creates the "Structure Box" on the page.
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{{STRUCTURE_2kyq| PDB=2kyq | SCENE= }}
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===1H, 15N, 13C chemical shifts and structure of CKR-brazzein===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 21765060 is the PubMed ID number.
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{{ABSTRACT_PUBMED_21765060}}
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==About this Structure==
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[[2kyq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pentadiplandra_brazzeana Pentadiplandra brazzeana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KYQ OCA].
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==Reference==
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<ref group="xtra">PMID:021765060</ref><references group="xtra"/>
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[[Category: Pentadiplandra brazzeana]]
[[Category: Pentadiplandra brazzeana]]
[[Category: Assadi-Porter, F M.]]
[[Category: Assadi-Porter, F M.]]

Revision as of 08:30, 30 April 2014

1H, 15N, 13C chemical shifts and structure of CKR-brazzein

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