2ib6

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==Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone cnidopus japonicus==
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[[Image:2ib6.png|left|200px]]
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<StructureSection load='2ib6' size='340' side='right' caption='[[2ib6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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[[2ib6]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IB6 OCA]. <br>
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<b>Related:</b> [[1mou|1mou]], [[1uis|1uis]], [[1g7k|1g7k]], [[1ema|1ema]], [[1xmz|1xmz]], [[2ib5|2ib5]]<br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/2ib6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
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Green fluorescent protein (GFP) and its relatives (GFP protein family) have been isolated from marine organisms such as jellyfish and corals that belong to the phylum Cnidaria (stinging aquatic invertebrates). They are intrinsically fluorescent proteins. In search of new members of the family of green fluorescent protein family, we identified a non-fluorescent chromoprotein from the Cnidopus japonicus species of sea anemone that possesses 45% sequence identity to dsRed (a red fluorescent protein). This newly identified blue color protein has an absorbance maximum of 610 nm and is hereafter referred to as cjBlue. Determination of the cjBlue 1.8 A crystal structure revealed a chromophore comprised of Gln(63)-Tyr(64)-Gly(65). The ring stacking between Tyr(64) and His(197) stabilized the cjBlue trans chromophore conformation along the Calpha2-Cbeta2 bond of 5-[(4-hydroxyphenyl)methylene]-imidazolinone, which closely resembled that of the "Kindling Fluorescent Protein" and Rtms5. Replacement of Tyr(64) with Leu in wild-type cjBlue produced a visible color change from blue to yellow with a new absorbance maximum of 417 nm. Interestingly, the crystal structure of the yellow mutant Y64L revealed two His(197) imidazole ring orientations, suggesting a flip-flop interconversion between the two conformations in solution. We conclude that the dynamics and structure of the chromophore are both essential for the optical appearance of these color proteins.
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Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus.,Chan MC, Karasawa S, Mizuno H, Bosanac I, Ho D, Prive GG, Miyawaki A, Ikura M J Biol Chem. 2006 Dec 8;281(49):37813-9. Epub 2006 Oct 6. PMID:17028187<ref>PMID:17028187</ref>
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The line below this paragraph, containing "STRUCTURE_2ib6", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_2ib6| PDB=2ib6 | SCENE= }}
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===Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone cnidopus japonicus===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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__TOC__
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The line below this paragraph, {{ABSTRACT_PUBMED_17028187}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 17028187 is the PubMed ID number.
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-->
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{{ABSTRACT_PUBMED_17028187}}
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==About this Structure==
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2IB6 is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Cnidopus_japonicus Cnidopus japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IB6 OCA].
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==Reference==
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<ref group="xtra">PMID:17028187</ref><references group="xtra"/>
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[[Category: Cnidopus japonicus]]
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[[Category: Bosanac, I.]]
[[Category: Bosanac, I.]]
[[Category: Chan, M C.Y.]]
[[Category: Chan, M C.Y.]]
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[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
[[Category: Yellow]]
[[Category: Yellow]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:14:22 2009''
 

Revision as of 08:34, 30 April 2014

Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone cnidopus japonicus

2ib6, resolution 2.00Å

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