2ipi

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[[Image:2ipi.png|left|200px]]
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==Crystal Structure of Aclacinomycin Oxidoreductase==
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<StructureSection load='2ipi' size='340' side='right' caption='[[2ipi]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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[[2ipi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_galilaeus Streptomyces galilaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IPI OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ip/2ipi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
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The crystal structure of aclacinomycin oxidoreductase (AknOx), a tailoring enzyme involved in the biosynthesis of the polyketide antibiotic aclacinomycin, was determined to 1.65 A resolution by multiwavelength anomalous diffraction using data from selenomethionine-substituted crystals. The crystals belong to space group P2(1), with unit-cell parameters a = 68.2, b = 264.5, c = 68.2 A, beta = 119 degrees . Analysis of the intensity statistics clearly showed the presence of pseudomerohedral twinning. The data set could also be indexed and scaled with an R(sym) of 0.072 in the orthorhombic space group C222(1) (unit-cell parameters a = 69.7, b = 117.5, c = 264.4 A), indicating the possibility of pseudomerohedral twinning along the diagonal between the monoclinic a and c directions. Refinement using this twin operator resulted in an R(free) of 24.2%. A monoclinic lattice with a = c and beta close to 120 degrees can emulate a hexagonal metric, with the possibility of a threefold twin operator along the b axis and three twin domains. Refinement assuming three-domain twinning gave a final R(free) of 26.5%. The structure of AknOx can be thus refined with comparable R(free) values using either of the twin operators separately, suggesting the possibility that crystals of AknOx contain six twin domains generated by the twofold and threefold twin operators perpendicular to each other. Both twin operators coincide with noncrystallographic symmetry axes that may promote twinning.
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Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidoreductase: indications of multidomain pseudomerohedral twinning.,Sultana A, Alexeev I, Kursula I, Mantsala P, Niemi J, Schneider G Acta Crystallogr D Biol Crystallogr. 2007 Feb;63(Pt 2):149-59. Epub 2007, Jan 16. PMID:17242508<ref>PMID:17242508</ref>
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The line below this paragraph, containing "STRUCTURE_2ipi", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_2ipi| PDB=2ipi | SCENE= }}
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===Crystal Structure of Aclacinomycin Oxidoreductase===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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<!--
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__TOC__
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The line below this paragraph, {{ABSTRACT_PUBMED_17242508}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 17242508 is the PubMed ID number.
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-->
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{{ABSTRACT_PUBMED_17242508}}
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==About this Structure==
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[[2ipi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_galilaeus Streptomyces galilaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IPI OCA].
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==Reference==
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<ref group="xtra">PMID:017242508</ref><ref group="xtra">PMID:017395717</ref><references group="xtra"/>
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[[Category: Streptomyces galilaeus]]
[[Category: Streptomyces galilaeus]]
[[Category: Alexeev, I.]]
[[Category: Alexeev, I.]]

Revision as of 08:34, 30 April 2014

Crystal Structure of Aclacinomycin Oxidoreductase

2ipi, resolution 1.65Å

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