2lgm

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[[Image:2lgm.png|left|200px]]
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==Structure of DNA Containing an Aristolactam II-dA Lesion==
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<StructureSection load='2lgm' size='340' side='right' caption='[[2lgm]], [[NMR_Ensembles_of_Models | 26 NMR models]]' scene=''>
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== Structural highlights ==
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[[2lgm]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LGM OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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Aristolochic acids I and II are prevalent plant toxicants found in the Aristolochiaceae plant family. Metabolic activation of the aristolochic acids leads to the formation of a cyclic N-hydroxylactam product that can react with the peripheral amino group of purine bases generating bulky DNA adducts. These lesions are mutagenic and established human carcinogens. Interestingly, although AL-dG adducts progressively disappear from the DNA of laboratory animals, AL-dA lesions has lasting persistence in the genome. We describe here NMR structural studies of an undecameric duplex damaged at its center by the presence of an ALII-dA adduct. Our data establish a locally perturbed double helical structure that accommodates the bulky adduct by displacing the counter residue into the major groove and stacking the ALII moiety between flanking bases. The presence of the ALII-dA perturbs the conformation of the 5'-side flanking base pair, but all other pairs of the duplex adopt standard conformations. Thermodynamic studies reveal that the lesion slightly decreases the energy of duplex formation in a sequence-dependent manner. We discuss our results in terms of its implications for the repair of ALII-dA adducts in mammalian cells.
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Structure and stability of DNA containing an aristolactam II-dA lesion: implications for the NER recognition of bulky adducts.,Lukin M, Zaliznyak T, Johnson F, de Los Santos C Nucleic Acids Res. 2012 Mar 1;40(6):2759-70. Epub 2011 Nov 25. PMID:22121223<ref>PMID:22121223</ref>
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The line below this paragraph, containing "STRUCTURE_2lgm", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2lgm| PDB=2lgm | SCENE= }}
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===Structure of DNA Containing an Aristolactam II-dA Lesion===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22121223}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 22121223 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22121223}}
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==About this Structure==
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[[2lgm]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LGM OCA].
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==Reference==
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<ref group="xtra">PMID:022121223</ref><references group="xtra"/>
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[[Category: Johnson, F.]]
[[Category: Johnson, F.]]
[[Category: Lukin, M.]]
[[Category: Lukin, M.]]

Revision as of 08:35, 30 April 2014

Structure of DNA Containing an Aristolactam II-dA Lesion

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