2lfs

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[[Image:2lfs.png|left|200px]]
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==Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer; A219F variant==
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<StructureSection load='2lfs' size='340' side='right' caption='[[2lfs]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''>
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== Structural highlights ==
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[[2lfs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LFS OCA]. <br>
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<b>Related:</b> [[3zrx|3zrx]], [[3zrw|3zrw]], [[3zrv|3zrv]], [[2lfr|2lfr]]<br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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Bacterial transmembrane receptors regulate an intracellular catalytic output in response to extracellular sensory input. To investigate the conformational changes that relay the regulatory signal, we have studied the HAMP domain, a ubiquitous intracellular module connecting input to output domains. HAMP forms a parallel, dimeric, four-helical coiled coil, and rational substitutions in our model domain (Af1503 HAMP) induce a transition in its interhelical packing, characterized by axial rotation of all four helices (the gearbox signaling model). We now illustrate how these conformational changes are propagated to a downstream domain by fusing Af1503 HAMP variants to the DHp domain of EnvZ, a bacterial histidine kinase. Structures of wild-type and mutant constructs are correlated with ligand response in vivo, clearly associating them with distinct signaling states. We propose that altered recognition of the catalytic domain by DHp, rather than a shift in position of the phospho-accepting histidine, forms the basis for regulation of kinase activity.
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Mechanism of regulation of receptor histidine kinases.,Ferris HU, Dunin-Horkawicz S, Hornig N, Hulko M, Martin J, Schultz JE, Zeth K, Lupas AN, Coles M Structure. 2012 Jan 11;20(1):56-66. PMID:22244755<ref>PMID:22244755</ref>
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The line below this paragraph, containing "STRUCTURE_2lfs", creates the "Structure Box" on the page.
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{{STRUCTURE_2lfs| PDB=2lfs | SCENE= }}
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===Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer; A219F variant===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_22244755}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 22244755 is the PubMed ID number.
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[[Category: Shigella paradysenteriae weldin 1927]]
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{{ABSTRACT_PUBMED_22244755}}
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==About this Structure==
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[[2lfs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus,_shigella_flexneri Archaeoglobus fulgidus, shigella flexneri]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LFS OCA].
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==Reference==
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<ref group="xtra">PMID:022244755</ref><references group="xtra"/>
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[[Category: Archaeoglobus fulgidus, shigella flexneri]]
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[[Category: Histidine kinase]]
[[Category: Histidine kinase]]
[[Category: Coles, M.]]
[[Category: Coles, M.]]

Revision as of 08:36, 30 April 2014

Solution structure of the chimeric Af1503 HAMP- EnvZ DHp homodimer; A219F variant

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