2jrg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2jrg" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2jrg.png|left|200px]]
+
==NMR solution structure of the anticodon of E. coli TRNA-VAL3 with 2 modifications (cmo5U34 M6A37)==
 +
<StructureSection load='2jrg' size='340' side='right' caption='[[2jrg]], [[NMR_Ensembles_of_Models | 11 NMR models]]' scene=''>
 +
== Structural highlights ==
 +
[[2jrg]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JRG OCA]. <br>
 +
<b>Related:</b> [[2jr4|2jr4]], [[2jrq|2jrq]]<br>
 +
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
 +
== Publication Abstract from PubMed ==
 +
The accuracy and efficiency with which tRNA decodes genomic information into proteins require posttranscriptional modifications in or adjacent to the anticodon. The modification uridine-5-oxyacetic acid (cmo (5)U 34) is found at wobble position 34 in a single isoaccepting tRNA species for six amino acids, alanine, leucine, proline, serine, threonine, and valine, each having 4-fold degenerate codons. cmo (5)U 34 makes possible the decoding of 24 codons by just six tRNAs. The contributions of this important modification to the structures and codon binding affinities of the unmodified and fully modified anticodon stem and loop domains of tRNA (Val3) UAC (ASL (Val3) UAC) were elucidated. The stems of the unmodified ASL (Val3) UAC and that with cmo (5)U 34 and N (6)-methyladenosine, m (6)A 37, adopted an A-form RNA conformation (rmsd approximately 0.6 A) as determined with NMR spectroscopy and torsion-angle molecular dynamics. However, the UV hyperchromicity, circular dichroism ellipticity, and structural analyses indicated that the anticodon modifications enhanced order in the loop. ASL (Val3) UAC-cmo (5)U 34;m (6)A 37 exhibited high affinities for its cognate and wobble codons GUA and GUG, and for GUU in the A-site of the programmed 30S ribosomal subunit, whereas the unmodified ASL (Val3) UAC bound less strongly to GUA and not at all to GUG and GUU. Together with recent crystal structures of ASL (Val3) UAC-cmo (5)U 34;m (6)A 37 bound to all four of the valine codons in the A-site of the ribosome's 30S subunit, these results clearly demonstrate that the xo (5)U 34-type modifications order the anticodon loop prior to A-site codon binding for an expanded codon reading, possibly reducing an entropic energy barrier to codon binding.
-
<!--
+
Anticodon domain modifications contribute order to tRNA for ribosome-mediated codon binding.,Vendeix FA, Dziergowska A, Gustilo EM, Graham WD, Sproat B, Malkiewicz A, Agris PF Biochemistry. 2008 Jun 10;47(23):6117-29. Epub 2008 May 13. PMID:18473483<ref>PMID:18473483</ref>
-
The line below this paragraph, containing "STRUCTURE_2jrg", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_2jrg| PDB=2jrg | SCENE= }}
+
-
===NMR solution structure of the anticodon of E. coli TRNA-VAL3 with 2 modifications (cmo5U34 M6A37)===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
== References ==
-
 
+
<references/>
-
<!--
+
__TOC__
-
The line below this paragraph, {{ABSTRACT_PUBMED_18473483}}, adds the Publication Abstract to the page
+
</StructureSection>
-
(as it appears on PubMed at http://www.pubmed.gov), where 18473483 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_18473483}}
+
-
 
+
-
==About this Structure==
+
-
[[2jrg]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JRG OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:018473483</ref><references group="xtra"/>
+
[[Category: Agris, P F.]]
[[Category: Agris, P F.]]
[[Category: Dziergowska, A.]]
[[Category: Dziergowska, A.]]

Revision as of 08:37, 30 April 2014

NMR solution structure of the anticodon of E. coli TRNA-VAL3 with 2 modifications (cmo5U34 M6A37)

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jrg"
Personal tools