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2l6m

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[[Image:2l6m.png|left|200px]]
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==Structure of C-terminal dsRBD of the Fission Yeast DICER (Dcr1)==
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<StructureSection load='2l6m' size='340' side='right' caption='[[2l6m]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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[[2l6m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L6M OCA]. <br>
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<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
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== Publication Abstract from PubMed ==
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Dicer proteins function in RNA interference (RNAi) pathways by generating small RNAs (sRNAs). Here, we report the solution structure of the C-terminal domain of Schizosaccharomyces pombe Dicer (Dcr1). The structure reveals an unusual double-stranded RNA binding domain (dsRBD) fold embedding a novel zinc-binding motif that is conserved among dicers in yeast. Although the C-terminal domain of Dcr1 still binds nucleic acids, this property is dispensable for proper functioning of Dcr1. In contrast, disruption of zinc coordination renders Dcr1 mainly cytoplasmic and leads to remarkable changes in gene expression and loss of heterochromatin assembly. In summary, our results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleocytoplasmic trafficking and not substrate binding. The C-terminal domain of Dcr1 constitutes a novel regulatory module that might represent a potential target for therapeutic intervention with fungal diseases.
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An extended dsRBD with a novel zinc-binding motif mediates nuclear retention of fission yeast Dicer.,Barraud P, Emmerth S, Shimada Y, Hotz HR, Allain FH, Buhler M EMBO J. 2011 Aug 16;30(20):4223-35. doi: 10.1038/emboj.2011.300. PMID:21847092<ref>PMID:21847092</ref>
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The line below this paragraph, containing "STRUCTURE_2l6m", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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{{STRUCTURE_2l6m| PDB=2l6m | SCENE= }}
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===Structure of C-terminal dsRBD of the Fission Yeast DICER (Dcr1)===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21847092}}, adds the Publication Abstract to the page
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</StructureSection>
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(as it appears on PubMed at http://www.pubmed.gov), where 21847092 is the PubMed ID number.
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{{ABSTRACT_PUBMED_21847092}}
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==About this Structure==
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[[2l6m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L6M OCA].
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==Reference==
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<ref group="xtra">PMID:021847092</ref><references group="xtra"/>
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[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Allain, F H.T.]]
[[Category: Allain, F H.T.]]

Revision as of 08:42, 30 April 2014

Structure of C-terminal dsRBD of the Fission Yeast DICER (Dcr1)

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