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4mzz
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(Difference between revisions)
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| - | + | ==Crystal structure of Bovine 3 Glu-Osteocalcin.== | |
| - | + | <StructureSection load='4mzz' size='340' side='right' caption='[[4mzz]], [[Resolution|resolution]] 1.88Å' scene=''> | |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4mzz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZZ OCA]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mzz RCSB], [http://www.ebi.ac.uk/pdbsum/4mzz PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The 3 Glu form of osteocalcin (3 Glu-OCN) is increased in serum during low vitamin K intake or oral anticoagulant use (warfarin). Previous reports using circular dichroism show it is less structured than 3 Gla Ca(2)(+)-osteocalcin and does not bind strongly to bone mineral. Recent studies have suggested a role for 3 Glu-OCN as a potential regulator of glucose metabolism. A G-protein-coupled receptor, GPRC6a, found in the pancreas and testes was identified as the putative osteocalcin receptor. The purpose of this study is to determine the high-resolution structure of bovine 3 Glu-OCN, using X-ray crystallography, to understand molecular interactions with mineral and the GPRC6a receptor. Diffraction quality crystals of thermally decarboxylated bovine osteocalcin were grown, and the crystal structure was determined to 1.88 A resolution. The final refined structure contained residues 17-47 and, like 3 Gla Ca(2)(+)-OCN, consisted of three alpha-helices surrounding a hydrophobic core, a C23-C29 disulfide bond between two of the helices, and no bound Ca(2)(+). Thus, the helical structure of 3 Glu-OCN is Ca(2)(+)-independent but similar to that of 3 Gla Ca(2)(+)-OCN. A reduced level of mineral binding could result from a lower number of Ca(2)(+) coordinating ligands on 3 Glu-OCN. The structure suggests the GPRC6a receptor may respond to helical osteocalcin and will aid in providing molecular mechanistic insight into the role of 3 Glu-OCN in glucose homeostasis. | ||
| - | + | X-ray crystal structure of bovine 3 Glu-osteocalcin.,Malashkevich VN, Almo SC, Dowd TL Biochemistry. 2013 Nov 26;52(47):8387-92. doi: 10.1021/bi4010254. Epub 2013 Nov, 12. PMID:24138653<ref>PMID:24138653</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[ | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bovin]] | ||
[[Category: Almo, S C.]] | [[Category: Almo, S C.]] | ||
[[Category: Dowd, T L.]] | [[Category: Dowd, T L.]] | ||
Revision as of 06:49, 7 May 2014
Crystal structure of Bovine 3 Glu-Osteocalcin.
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