2maw
From Proteopedia
(Difference between revisions)
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| - | + | ==NMR structures of the alpha7 nAChR transmembrane domain.== | |
| - | + | <StructureSection load='2maw' size='340' side='right' caption='[[2maw]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | ==Disease== | + | <table><tr><td colspan='2'>[[2maw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAW OCA]. <br> |
| + | </td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CHRFAM7A, CHRNA7, NACHRA7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2maw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2maw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2maw RCSB], [http://www.ebi.ac.uk/pdbsum/2maw PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Disease == | ||
[[http://www.uniprot.org/uniprot/ACHA7_HUMAN ACHA7_HUMAN]] 15q13.3 microdeletion syndrome. | [[http://www.uniprot.org/uniprot/ACHA7_HUMAN ACHA7_HUMAN]] 15q13.3 microdeletion syndrome. | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ACHA7_HUMAN ACHA7_HUMAN]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nicotinic acetylcholine receptors (nAChRs) are targets of general anesthetics, but functional sensitivity to anesthetic inhibition varies dramatically among different subtypes of nAChRs. Potential causes underlying different functional responses to anesthetics remain elusive. Here we show that in contrast to the alpha7 nAChR, the alpha7beta2 nAChR is highly susceptible to inhibition by the volatile anesthetic isoflurane in electrophysiology measurements. Isoflurane-binding sites in beta2 and alpha7 were found at the extracellular and intracellular end of their respective transmembrane domains using NMR. Functional relevance of the identified beta2 site was validated via point mutations and subsequent functional measurements. Consistent with their functional responses to isoflurane, beta2 but not alpha7 showed pronounced dynamics changes, particularly for the channel gate residue Leu-249(9'). These results suggest that anesthetic binding alone is not sufficient to generate functional impact; only those sites that can modulate channel dynamics upon anesthetic binding will produce functional effects. | ||
| - | + | Insights into distinct modulation of alpha7 and alpha7beta2 nicotinic acetylcholine receptors by the volatile anesthetic isoflurane.,Mowrey DD, Liu Q, Bondarenko V, Chen Q, Seyoum E, Xu Y, Wu J, Tang P J Biol Chem. 2013 Dec 13;288(50):35793-800. doi: 10.1074/jbc.M113.508333. Epub, 2013 Nov 5. PMID:24194515<ref>PMID:24194515</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[ | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Bondarenko, V.]] | [[Category: Bondarenko, V.]] | ||
[[Category: Mowrey, D.]] | [[Category: Mowrey, D.]] | ||
Revision as of 06:56, 7 May 2014
NMR structures of the alpha7 nAChR transmembrane domain.
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