This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2c0h
From Proteopedia
| Line 29: | Line 29: | ||
[[Category: tim alpha/beta barrel]] | [[Category: tim alpha/beta barrel]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:53:52 2007'' |
Revision as of 14:49, 30 October 2007
|
X-RAY STRUCTURE OF BETA-MANNANASE FROM BLUE MUSSEL MYTILUS EDULIS
Overview
Endo-beta-1,4-d-mannanase is the key depolymerizing enzyme for, beta-1,4-mannan polymers present in the cell walls of plants and some, algae, as well as in some types of plant seeds. Endo-1,4-beta-mannanase, from blue mussel Mytilus edulis (MeMan5A) belongs to the glycoside, hydrolase (GH) family 5 enzymes. The MeMan5A structure has been determined, to 1.6A resolution using the multiple-wavelength anomalous dispersion, method at the selenium K edge with selenomethionyl MeMan5A expressed in, the yeast Pichia pastoris. As expected for GH 5 enzymes, the structure, showed a (betaalpha)(8)-barrel fold. An unusually large number of, histidine side-chains are exposed on the surface, which may relate to its, location within the crystalline style of the digestive tract of the, mussel. Kinetic ... [(full description)]
About this Structure
2C0H is a [Single protein] structure of sequence from [Mytilus edulis] with SO4 as [ligand]. Active as [Mannan endo-1,4-beta-mannosidase], with EC number [3.2.1.78]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Three-dimensional crystal structure and enzymic characterization of beta-mannanase Man5A from blue mussel Mytilus edulis., Larsson AM, Anderson L, Xu B, Munoz IG, Uson I, Janson JC, Stalbrand H, Stahlberg J, J Mol Biol. 2006 Apr 14;357(5):1500-10. Epub 2006 Jan 31. PMID:16487541
Page seeded by OCA on Tue Oct 30 16:53:52 2007
