2w7z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2w7z" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2w7z.png|left|200px]]
+
==STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.==
 +
<StructureSection load='2w7z' size='340' side='right' caption='[[2w7z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2w7z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LP6:6-PIPERIDIN-1-YL-L-NORLEUCINE'>LP6</scene></td></tr>
 +
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w7z RCSB], [http://www.ebi.ac.uk/pdbsum/2w7z PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/2w7z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.
-
<!--
+
Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.,Vetting MW, Hegde SS, Blanchard JS Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):462-9. Epub 2009, Apr 18. PMID:19390151<ref>PMID:19390151</ref>
-
The line below this paragraph, containing "STRUCTURE_2w7z", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_2w7z| PDB=2w7z | SCENE= }}
+
-
===STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
 
+
== References ==
-
<!--
+
<references/>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19390151}}, adds the Publication Abstract to the page
+
__TOC__
-
(as it appears on PubMed at http://www.pubmed.gov), where 19390151 is the PubMed ID number.
+
</StructureSection>
-
-->
+
-
{{ABSTRACT_PUBMED_19390151}}
+
-
 
+
-
==About this Structure==
+
-
[[2w7z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:019390151</ref><references group="xtra"/>
+
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
[[Category: Blanchard, J S.]]
[[Category: Blanchard, J S.]]

Revision as of 08:39, 7 May 2014

STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.

2w7z, resolution 1.60Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w7z"
Views
Personal tools
Navigation
Toolbox