2wsj

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[[Image:2wsj.png|left|200px]]
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==CRYSTAL STRUCTURE OF SINGLE POINT MUTANT GLU71SER P-COUMARIC ACID DECARBOXYLASE==
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<StructureSection load='2wsj' size='340' side='right' caption='[[2wsj]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wsj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSJ OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w2b|2w2b]], [[2w2a|2w2a]], [[2w2f|2w2f]], [[2gc9|2gc9]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wsj RCSB], [http://www.ebi.ac.uk/pdbsum/2wsj PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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p-Coumaric acid decarboxylases (PDCs) catalyze the nonoxidative decarboxylation of hydroxycinnamic acids to generate the corresponding vinyl derivatives. Despite the biotechnological relevance of PDCs in food industry, their catalytic mechanism remains largely unknown. Here, we report insights into the structural basis of catalysis for the homodimeric PDC from Lactobacillus plantarum (LpPDC). The global fold of LpPDC is based on a flattened beta-barrel surrounding an internal cavity. Crystallographic and functional analyses of single-point mutants of residues located within this cavity have permitted identifying a potential substrate-binding pocket and also to provide structural evidences for rearrangements of surface loops so that they can modulate the accessibility to the active site. Finally, combination of the structural and functional data with in silico results enables us to propose a two-step catalytic mechanism for decarboxylation of p-coumaric acid by PDCs where Glu71 is involved in proton transfer, and Tyr18 and Tyr20 are involved in the proper substrate orientation and in the release of the CO(2) product. Proteins 2010. (c) 2010 Wiley-Liss, Inc.
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<!--
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p-Coumaric acid decarboxylase from Lactobacillus plantarum: Structural insights into the active site and decarboxylation catalytic mechanism.,Rodriguez H, Angulo I, de Las Rivas B, Campillo N, Paez JA, Munoz R, Mancheno JM Proteins. 2009 Dec 21. PMID:20112419<ref>PMID:20112419</ref>
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The line below this paragraph, containing "STRUCTURE_2wsj", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_2wsj| PDB=2wsj | SCENE= }}
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===CRYSTAL STRUCTURE OF SINGLE POINT MUTANT GLU71SER P-COUMARIC ACID DECARBOXYLASE===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_20112419}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 20112419 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_20112419}}
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==About this Structure==
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[[2wsj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSJ OCA].
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==Reference==
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<ref group="xtra">PMID:020112419</ref><references group="xtra"/>
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[[Category: Lactobacillus plantarum]]
[[Category: Lactobacillus plantarum]]
[[Category: Angulo, I.]]
[[Category: Angulo, I.]]

Revision as of 08:39, 7 May 2014

CRYSTAL STRUCTURE OF SINGLE POINT MUTANT GLU71SER P-COUMARIC ACID DECARBOXYLASE

2wsj, resolution 2.24Å

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