2wbn

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[[Image:2wbn.png|left|200px]]
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==CRYSTAL STRUCTURE OF THE G2P (LARGE TERMINASE) NUCLEASE DOMAIN FROM THE BACTERIOPHAGE SPP1==
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<StructureSection load='2wbn' size='340' side='right' caption='[[2wbn]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wbn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spp1 Bacillus phage spp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WBN OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wc9|2wc9]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wbn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wbn RCSB], [http://www.ebi.ac.uk/pdbsum/2wbn PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/2wbn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DNA-packaging motor in tailed bacteriophages requires nuclease activity to ensure that the genome is packaged correctly. This nuclease activity is tightly regulated as the enzyme is inactive for the duration of DNA translocation. Here, we report the X-ray structure of the large terminase nuclease domain from bacteriophage SPP1. Similarity with the RNase H family endonucleases allowed interactions with the DNA to be predicted. A structure-based alignment with the distantly related T4 gp17 terminase shows the conservation of an extended beta-sheet and an auxiliary beta-hairpin that are not found in other RNase H family proteins. The model with DNA suggests that the beta-hairpin partly blocks the active site, and in vivo activity assays show that the nuclease domain is not functional in the absence of the ATPase domain. Here, we propose that the nuclease activity is regulated by movement of the beta-hairpin, altering active site access and the orientation of catalytically essential residues.
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Structural basis for the nuclease activity of a bacteriophage large terminase.,Smits C, Chechik M, Kovalevskiy OV, Shevtsov MB, Foster AW, Alonso JC, Antson AA EMBO Rep. 2009 Jun;10(6):592-8. Epub 2009 May 15. PMID:19444313<ref>PMID:19444313</ref>
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The line below this paragraph, containing "STRUCTURE_2wbn", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_2wbn| PDB=2wbn | SCENE= }}
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===CRYSTAL STRUCTURE OF THE G2P (LARGE TERMINASE) NUCLEASE DOMAIN FROM THE BACTERIOPHAGE SPP1===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_19444313}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 19444313 is the PubMed ID number.
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</StructureSection>
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-->
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{{ABSTRACT_PUBMED_19444313}}
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==About this Structure==
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[[2wbn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_spp1 Bacillus phage spp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WBN OCA].
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==Reference==
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<ref group="xtra">PMID:019444313</ref><references group="xtra"/>
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[[Category: Bacillus phage spp1]]
[[Category: Bacillus phage spp1]]
[[Category: Alonso, J C.]]
[[Category: Alonso, J C.]]

Revision as of 08:43, 7 May 2014

CRYSTAL STRUCTURE OF THE G2P (LARGE TERMINASE) NUCLEASE DOMAIN FROM THE BACTERIOPHAGE SPP1

2wbn, resolution 1.90Å

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OCA

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