2y95

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[[Image:2y95.png|left|200px]]
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==Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation==
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<StructureSection load='2y95' size='340' side='right' caption='[[2y95]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y95 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y95 RCSB], [http://www.ebi.ac.uk/pdbsum/2y95 PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors.
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The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization.,Duszczyk MM, Wutz A, Rybin V, Sattler M RNA. 2011 Nov;17(11):1973-82. Epub 2011 Sep 26. PMID:21947263<ref>PMID:21947263</ref>
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The line below this paragraph, containing "STRUCTURE_2y95", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2y95| PDB=2y95 | SCENE= }}
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===Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21947263}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21947263 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21947263}}
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==About this Structure==
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[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA].
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==Reference==
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<ref group="xtra">PMID:021947263</ref><ref group="xtra">PMID:021779925</ref><references group="xtra"/>
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[[Category: Duszczyk, M M.]]
[[Category: Duszczyk, M M.]]
[[Category: Sattler, M.]]
[[Category: Sattler, M.]]

Revision as of 07:42, 14 May 2014

Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation

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