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2xt0

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[[Image:2xt0.png|left|200px]]
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==DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I==
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<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A haloalkane dehalogenase (DppA) from Plesiocystis pacifica SIR-1 was identified by sequence comparison in the NCBI database, cloned, functionally expressed in Escherichia coli, purified, and biochemically characterized. The three-dimensional (3D) structure was determined by X-ray crystallography and has been refined at 1.95 A resolution to an R-factor of 21.93%. The enzyme is composed of an alpha/beta-hydrolase fold and a cap domain and the overall fold is similar to other known haloalkane dehalogenases. Active site residues were identified as Asp123, His278, and Asp249 and Trp124 and Trp163 as halide-stabilizing residues. DppA, like DhlA from Xanthobacter autotrophicus GJ10, is a member of the haloalkane dehalogenase subfamily HLD-I. As a consequence, these enzymes have in common the relative position of their catalytic residues within the structure and also show some similarities in the substrate specificity. The enzyme shows high preference for 1-bromobutane and does not accept chlorinated alkanes, halo acids, or halo alcohols. It is a monomeric protein with a molecular mass of 32.6 kDa and exhibits maximum activity between 33 and 37 degrees C with a pH optimum between pH 8 and 9. The K(m) and k(cat) values for 1-bromobutane were 24.0 mM and 8.08 s(-1). Furthermore, from the 3D-structure of DppA, it was found that the enzyme possesses a large and open active site pocket. Docking experiments were performed to explain the experimentally determined substrate preferences.
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Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1.,Hesseler M, Bogdanovic X, Hidalgo A, Berenguer J, Palm GJ, Hinrichs W, Bornscheuer UT Appl Microbiol Biotechnol. 2011 Aug;91(4):1049-60. Epub 2011 May 21. PMID:21603934<ref>PMID:21603934</ref>
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The line below this paragraph, containing "STRUCTURE_2xt0", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2xt0| PDB=2xt0 | SCENE= }}
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===DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21603934}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21603934 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21603934}}
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==About this Structure==
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[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA].
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==Reference==
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<ref group="xtra">PMID:021603934</ref><ref group="xtra">PMID:020606284</ref><references group="xtra"/>
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[[Category: Haloalkane dehalogenase]]
[[Category: Haloalkane dehalogenase]]
[[Category: Plesiocystis pacifica]]
[[Category: Plesiocystis pacifica]]

Revision as of 07:44, 14 May 2014

DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I

2xt0, resolution 1.90Å

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