2xse

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[[Image:2xse.png|left|200px]]
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==THE STRUCTURAL BASIS FOR RECOGNITION OF J-BASE CONTAINING DNA BY A NOVEL DNA-BINDING DOMAIN IN JBP1==
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<StructureSection load='2xse' size='340' side='right' caption='[[2xse]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSE OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xse RCSB], [http://www.ebi.ac.uk/pdbsum/2xse PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The J-binding protein 1 (JBP1) is essential for biosynthesis and maintenance of DNA base-J (beta-d-glucosyl-hydroxymethyluracil). Base-J and JBP1 are confined to some pathogenic protozoa and are absent from higher eukaryotes, prokaryotes and viruses. We show that JBP1 recognizes J-containing DNA (J-DNA) through a 160-residue domain, DB-JBP1, with 10 000-fold preference over normal DNA. The crystal structure of DB-JBP1 revealed a helix-turn-helix variant fold, a 'helical bouquet' with a 'ribbon' helix encompassing the amino acids responsible for DNA binding. Mutation of a single residue (Asp525) in the ribbon helix abrogates specificity toward J-DNA. The same mutation renders JBP1 unable to rescue the targeted deletion of endogenous JBP1 genes in Leishmania and changes its distribution in the nucleus. Based on mutational analysis and hydrogen/deuterium-exchange mass-spectrometry data, a model of JBP1 bound to J-DNA was constructed and validated by small-angle X-ray scattering data. Our results open new possibilities for targeted prevention of J-DNA recognition as a therapeutic intervention for parasitic diseases.
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The structural basis for recognition of base J containing DNA by a novel DNA binding domain in JBP1.,Heidebrecht T, Christodoulou E, Chalmers MJ, Jan S, Ter Riet B, Grover RK, Joosten RP, Littler D, van Luenen H, Griffin PR, Wentworth P Jr, Borst P, Perrakis A Nucleic Acids Res. 2011 Mar 16. PMID:21415010<ref>PMID:21415010</ref>
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The line below this paragraph, containing "STRUCTURE_2xse", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_2xse| PDB=2xse | SCENE= }}
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===THE STRUCTURAL BASIS FOR RECOGNITION OF J-BASE CONTAINING DNA BY A NOVEL DNA-BINDING DOMAIN IN JBP1===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21415010}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21415010 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21415010}}
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==About this Structure==
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[[2xse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSE OCA].
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==Reference==
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<ref group="xtra">PMID:021415010</ref><references group="xtra"/>
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[[Category: Leishmania tarentolae]]
[[Category: Leishmania tarentolae]]
[[Category: Thymine dioxygenase]]
[[Category: Thymine dioxygenase]]

Revision as of 07:46, 14 May 2014

THE STRUCTURAL BASIS FOR RECOGNITION OF J-BASE CONTAINING DNA BY A NOVEL DNA-BINDING DOMAIN IN JBP1

2xse, resolution 1.90Å

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