2x9p

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2x9p" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2x9p.png|left|200px]]
+
==X-RAY STRUCTURE OF THE SUBSTRATE-FREE CYTOCHROME P450 PIMD -A POLYENE MACROLIDE ANTIBIOTIC PIMARICIN EPOXIDASE==
 +
<StructureSection load='2x9p' size='340' side='right' caption='[[2x9p]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2x9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_natalensis Streptomyces natalensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9P OCA]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xbk|2xbk]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x9p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x9p RCSB], [http://www.ebi.ac.uk/pdbsum/2x9p PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x9/2x9p_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We present the X-ray structure of PimD, both substrate-free and in complex with 4,5-desepoxypimaricin. PimD is a cytochrome P450 monooxygenase with native epoxidase activity that is critical in the biosynthesis of the polyene macrolide antibiotic pimaricin. Intervention in this secondary metabolic pathway could advance the development of drugs with improved pharmacologic properties. Epoxidation by P450 typically includes formation of a charge-transfer complex between an oxoferryl pi-cation radical species (Compound I) and the olefin pi-bond as the initial intermediate. Catalytic and structural evidence presented here suggest that epoxidation of 4,5-desepoxypimaricin proceeds via a hydroperoxoferric intermediate (Compound 0). The oxygen atom of Compound 0 distal to the heme iron may insert into the double bond of the substrate to make an epoxide ring. Stereoelectronic features of the putative transition state suggest substrate-assisted proton delivery.
-
<!--
+
Structure of cytochrome P450 PimD suggests epoxidation of the polyene macrolide pimaricin occurs via a hydroperoxoferric intermediate.,Kells PM, Ouellet H, Santos-Aberturas J, Aparicio JF, Podust LM Chem Biol. 2010 Aug 27;17(8):841-51. PMID:20797613<ref>PMID:20797613</ref>
-
The line below this paragraph, containing "STRUCTURE_2x9p", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
-->
+
-
{{STRUCTURE_2x9p| PDB=2x9p | SCENE= }}
+
-
===X-RAY STRUCTURE OF THE SUBSTRATE-FREE CYTOCHROME P450 PIMD -A POLYENE MACROLIDE ANTIBIOTIC PIMARICIN EPOXIDASE===
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
 
+
== References ==
-
<!--
+
<references/>
-
The line below this paragraph, {{ABSTRACT_PUBMED_20797613}}, adds the Publication Abstract to the page
+
__TOC__
-
(as it appears on PubMed at http://www.pubmed.gov), where 20797613 is the PubMed ID number.
+
</StructureSection>
-
-->
+
-
{{ABSTRACT_PUBMED_20797613}}
+
-
 
+
-
==About this Structure==
+
-
[[2x9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_natalensis Streptomyces natalensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X9P OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:020797613</ref><references group="xtra"/>
+
[[Category: Streptomyces natalensis]]
[[Category: Streptomyces natalensis]]
[[Category: Aparicio, J F.]]
[[Category: Aparicio, J F.]]

Revision as of 07:49, 14 May 2014

X-RAY STRUCTURE OF THE SUBSTRATE-FREE CYTOCHROME P450 PIMD -A POLYENE MACROLIDE ANTIBIOTIC PIMARICIN EPOXIDASE

2x9p, resolution 2.10Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2x9p"
Views
Personal tools
Navigation
Toolbox