2yjy

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[[Image:2yjy.png|left|200px]]
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==A SPECIFIC AND MODULAR BINDING CODE FOR CYTOSINE RECOGNITION IN PUF DOMAINS==
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<StructureSection load='2yjy' size='340' side='right' caption='[[2yjy]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2yjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJY OCA]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ib2|1ib2]], [[1m8y|1m8y]], [[1m8z|1m8z]], [[1m8w|1m8w]], [[1m8x|1m8x]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjy RCSB], [http://www.ebi.ac.uk/pdbsum/2yjy PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pumilio/fem-3 mRNA-binding factor (PUF) proteins possess a recognition code for bases A, U, and G, allowing designed RNA sequence specificity of their modular Pumilio (PUM) repeats. However, recognition side chains in a PUM repeat for cytosine are unknown. Here we report identification of a cytosine-recognition code by screening random amino acid combinations at conserved RNA recognition positions using a yeast three-hybrid system. This C-recognition code is specific and modular as specificity can be transferred to different positions in the RNA recognition sequence. A crystal structure of a modified PUF domain reveals specific contacts between an arginine side chain and the cytosine base. We applied the C-recognition code to design PUF domains that recognize targets with multiple cytosines and to generate engineered splicing factors that modulate alternative splicing. Finally, we identified a divergent yeast PUF protein, Nop9p, that may recognize natural target RNAs with cytosine. This work deepens our understanding of natural PUF protein target recognition and expands the ability to engineer PUF domains to recognize any RNA sequence.
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Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains.,Dong S, Wang Y, Cassidy-Amstutz C, Lu G, Bigler R, Jezyk MR, Li C, Hall TM, Wang Z J Biol Chem. 2011 Jul 29;286(30):26732-42. Epub 2011 Jun 8. PMID:21653694<ref>PMID:21653694</ref>
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The line below this paragraph, containing "STRUCTURE_2yjy", creates the "Structure Box" on the page.
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{{STRUCTURE_2yjy| PDB=2yjy | SCENE= }}
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===A SPECIFIC AND MODULAR BINDING CODE FOR CYTOSINE RECOGNITION IN PUF DOMAINS===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21653694}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21653694 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21653694}}
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==About this Structure==
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[[2yjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJY OCA].
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==Reference==
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<ref group="xtra">PMID:021653694</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Bigler, R.]]
[[Category: Bigler, R.]]

Revision as of 07:52, 14 May 2014

A SPECIFIC AND MODULAR BINDING CODE FOR CYTOSINE RECOGNITION IN PUF DOMAINS

2yjy, resolution 2.60Å

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