3awy

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[[Image:3awy.png|left|200px]]
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==Crystal structure of Streptomyces tyrosinase in a complex with caddie M84L mutant soaked in a Cu(II)-containing solution for 80 hr==
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<StructureSection load='3awy' size='340' side='right' caption='[[3awy]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3awy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_castaneoglobisporus Streptomyces castaneoglobisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AWY FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aws|3aws]], [[3awt|3awt]], [[3awu|3awu]], [[3awv|3awv]], [[3aww|3aww]], [[3awx|3awx]], [[3awz|3awz]], [[3ax0|3ax0]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TYRC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=79261 Streptomyces castaneoglobisporus]), ORF378 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=79261 Streptomyces castaneoglobisporus])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Monophenol_monooxygenase Monophenol monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.18.1 1.14.18.1] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3awy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3awy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3awy RCSB], [http://www.ebi.ac.uk/pdbsum/3awy PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Cu(II)-soaked crystal structure of tyrosinase that is present in a complex with a protein, designated "caddie," which we previously determined, possesses two copper ions at its catalytic center. We had identified two copper-binding sites in the caddie protein and speculated that copper bound to caddie may be transported to the tyrosinase catalytic center. In our present study, at a 1.16-1.58 A resolution, we determined the crystal structures of tyrosinase complexed with caddie prepared by altering the soaking time of the copper ion and the structures of tyrosinase complexed with different caddie mutants that display little or no capacity to activate tyrosinase. Based on these structures, we propose a molecular mechanism by which two copper ions are transported to the tyrosinase catalytic center with the assistance of caddie acting as a metallochaperone.
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A molecular mechanism for copper transportation to tyrosinase that is assisted by a metallochaperone, caddie protein.,Matoba Y, Bando N, Oda K, Noda M, Higashikawa F, Kumagai T, Sugiyama M J Biol Chem. 2011 Aug 26;286(34):30219-31. Epub 2011 Jul 5. PMID:21730070<ref>PMID:21730070</ref>
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The line below this paragraph, containing "STRUCTURE_3awy", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3awy| PDB=3awy | SCENE= }}
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===Crystal structure of Streptomyces tyrosinase in a complex with caddie M84L mutant soaked in a Cu(II)-containing solution for 80 hr===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21730070}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21730070 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_21730070}}
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==About this Structure==
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[[3awy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_castaneoglobisporus Streptomyces castaneoglobisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AWY OCA].
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==Reference==
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<ref group="xtra">PMID:021730070</ref><ref group="xtra">PMID:016436386</ref><references group="xtra"/>
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[[Category: Monophenol monooxygenase]]
[[Category: Monophenol monooxygenase]]
[[Category: Streptomyces castaneoglobisporus]]
[[Category: Streptomyces castaneoglobisporus]]

Revision as of 11:06, 14 May 2014

Crystal structure of Streptomyces tyrosinase in a complex with caddie M84L mutant soaked in a Cu(II)-containing solution for 80 hr

3awy, resolution 1.58Å

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