192d
From Proteopedia
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| - | [[Image:192d.gif|left|200px]] | + | [[Image:192d.gif|left|200px]] |
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| - | '''RECOMBINATION-LIKE STRUCTURE OF D(CCGCGG)''' | + | {{Structure |
| + | |PDB= 192d |SIZE=350|CAPTION= <scene name='initialview01'>192d</scene>, resolution 1.920Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''RECOMBINATION-LIKE STRUCTURE OF D(CCGCGG)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 192D is a [ | + | 192D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=192D OCA]. |
==Reference== | ==Reference== | ||
| - | Recombination-like structure of d(CCGCGG)., Malinina L, Urpi L, Salas X, Huynh-Dinh T, Subirana JA, J Mol Biol. 1994 Oct 28;243(3):484-93. PMID:[http:// | + | Recombination-like structure of d(CCGCGG)., Malinina L, Urpi L, Salas X, Huynh-Dinh T, Subirana JA, J Mol Biol. 1994 Oct 28;243(3):484-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7966274 7966274] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Huynh-Dinh, T.]] | [[Category: Huynh-Dinh, T.]] | ||
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[[Category: NA]] | [[Category: NA]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | [[Category: flipped-out | + | [[Category: flipped-out base]] |
[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:50:43 2008'' |
Revision as of 07:50, 20 March 2008
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| , resolution 1.920Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RECOMBINATION-LIKE STRUCTURE OF D(CCGCGG)
Overview
We have solved the single crystal X-ray structure of the synthetic DNA hexamer d(CCGCGG). The central alternating tetramer forms a Z-DNA duplex. The initial cytosine of each strand of the duplex swings out and forms a Watson-Crick base-pair with the terminal guanine of a symmetry-related molecule. Thus, two symmetry-related DNA molecules form a twin with intermolecular base-pairs at both ends. Such a twin is additionally stabilized by a sodium ion located on a dyad axis between two DNA duplexes. The total structure has recombination-like features. It also provides a model for B/Z junctions. The crystal used in this study belongs to space group C222(1) with a = 34.33 A, b = 44.04 A and c = 38.27 A. The structure was solved by molecular replacement using partial models, and refined by molecular dynamics simulated annealing and positional treatment. The refinement has been concluded with an R-factor of 18.5% for 2377 reflections with F > or = 2 sigma (F) in the resolution region 8.0 to 1.92 A. The asymmetric unit contains two strands of d(CCGCGG) and 38 water molecules.
About this Structure
192D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Recombination-like structure of d(CCGCGG)., Malinina L, Urpi L, Salas X, Huynh-Dinh T, Subirana JA, J Mol Biol. 1994 Oct 28;243(3):484-93. PMID:7966274
Page seeded by OCA on Thu Mar 20 09:50:43 2008
