1a48
From Proteopedia
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| - | [[Image:1a48.gif|left|200px]] | + | [[Image:1a48.gif|left|200px]] |
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| - | '''SAICAR SYNTHASE''' | + | {{Structure |
| + | |PDB= 1a48 |SIZE=350|CAPTION= <scene name='initialview01'>1a48</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoribosylaminoimidazolesuccinocarboxamide_synthase Phosphoribosylaminoimidazolesuccinocarboxamide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.6 6.3.2.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SAICAR SYNTHASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A48 is a [ | + | 1A48 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A48 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis., Levdikov VM, Barynin VV, Grebenko AI, Melik-Adamyan WR, Lamzin VS, Wilson KS, Structure. 1998 Mar 15;6(3):363-76. PMID:[http:// | + | The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis., Levdikov VM, Barynin VV, Grebenko AI, Melik-Adamyan WR, Lamzin VS, Wilson KS, Structure. 1998 Mar 15;6(3):363-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9551557 9551557] |
[[Category: Phosphoribosylaminoimidazolesuccinocarboxamide synthase]] | [[Category: Phosphoribosylaminoimidazolesuccinocarboxamide synthase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: purine biosynthesis]] | [[Category: purine biosynthesis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:52:38 2008'' |
Revision as of 07:52, 20 March 2008
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| , resolution 1.9Å | |||||||
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| Ligands: | and | ||||||
| Activity: | Phosphoribosylaminoimidazolesuccinocarboxamide synthase, with EC number 6.3.2.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SAICAR SYNTHASE
Overview
BACKGROUND: The biosynthesis of key metabolic components is of major interest to biologists. Studies of de novo purine synthesis are aimed at obtaining a deeper understanding of this central pathway and the development of effective chemotherapeutic agents. Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase catalyses the seventh step out of ten in the biosynthesis of purine nucleotides. To date, only one structure of an enzyme involved in purine biosynthesis has been reported: adenylosuccinate synthetase, which catalyses the first committed step in the synthesis of AMP from IMP. RESULTS: We report the first three-dimensional structure of a SAICAR synthase, from Saccharomyces cerevisiae. It is a monomer with three domains. The first two domains consist of antiparallel beta sheets and the third is composed of two alpha helices. There is a long deep cleft made up of residues from all three domains. Comparison of SAICAR synthases by alignment of their sequences reveals a number of conserved residues, mostly located in the cleft. The presence of two sulphate ions bound in the cleft, the structure of SAICAR synthase in complex with ATP and a comparison of this structure with that of other ATP-dependent proteins point to the interdomain cleft as the location of the active site. CONCLUSIONS: The topology of the first domain of SAICAR synthase resembles that of the N-terminal domain of proteins belonging to the cyclic AMP-dependent protein kinase family. The fold of the second domain is similar to that of members of the D-alanine:D-alanine ligase family. Together these enzymes form a new superfamily of mononucleotide-binding domains. There appears to be no other enzyme, however, which is composed of the same combination of three domains, with the individual topologies found in SAICAR synthase.
About this Structure
1A48 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis., Levdikov VM, Barynin VV, Grebenko AI, Melik-Adamyan WR, Lamzin VS, Wilson KS, Structure. 1998 Mar 15;6(3):363-76. PMID:9551557
Page seeded by OCA on Thu Mar 20 09:52:38 2008
Categories: Phosphoribosylaminoimidazolesuccinocarboxamide synthase | Saccharomyces cerevisiae | Single protein | Lamzin, V S. | Levdikov, V M. | Melik-Adamyan, W R. | Wilson, K S. | ACE | SO4 | Atp binding protein | Crystal structure | Phosphoribosylaminoimidazolesuccinocarboxamide (saicar) synthase | Purine biosynthesis
