1a4t
From Proteopedia
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| - | [[Image:1a4t.jpg|left|200px]] | + | [[Image:1a4t.jpg|left|200px]] |
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| - | '''SOLUTION STRUCTURE OF PHAGE P22 N PEPTIDE-BOX B RNA COMPLEX, NMR, 20 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1a4t |SIZE=350|CAPTION= <scene name='initialview01'>1a4t</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''SOLUTION STRUCTURE OF PHAGE P22 N PEPTIDE-BOX B RNA COMPLEX, NMR, 20 STRUCTURES''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A4T is a [ | + | 1A4T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_phage_py54 Yersinia phage py54]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A4T OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of P22 transcriptional antitermination N peptide-boxB RNA complex., Cai Z, Gorin A, Frederick R, Ye X, Hu W, Majumdar A, Kettani A, Patel DJ, Nat Struct Biol. 1998 Mar;5(3):203-12. PMID:[http:// | + | Solution structure of P22 transcriptional antitermination N peptide-boxB RNA complex., Cai Z, Gorin A, Frederick R, Ye X, Hu W, Majumdar A, Kettani A, Patel DJ, Nat Struct Biol. 1998 Mar;5(3):203-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9501914 9501914] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Yersinia phage py54]] | [[Category: Yersinia phage py54]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:52:53 2008'' |
Revision as of 07:52, 20 March 2008
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SOLUTION STRUCTURE OF PHAGE P22 N PEPTIDE-BOX B RNA COMPLEX, NMR, 20 STRUCTURES
Overview
We have determined the solution structure of a 15-mer boxB RNA hairpin complexed with a 20-mer basic peptide of the N protein involved in bacteriophage P22 transcriptional antitermination. Complex formation involves adaptive binding with the N peptide adopting a bent alpha-helical conformation that packs tightly through hydrophobic and electrostatic interactions against the major groove face of the boxB RNA hairpin, orienting the open opposite face for potential interactions with host factors and/or RNA polymerase. Four nucleotides in the boxB RNA hairpin pentaloop form a stable GNRA like tetraloop structural scaffold on complex formation, allowing the looped out fifth nucleotide to make extensive hydrophobic contacts with the bound peptide. The guanidinium group of a key arginine is hydrogen-bonded to the guanine in a loop-closing sheared G.A mismatch and to adjacent backbone phosphates. The identified intermolecular contacts account for the consequences of N peptide and boxB RNA mutations on bacteriophage transcriptional antitermination.
About this Structure
1A4T is a Single protein structure of sequence from Yersinia phage py54. Full crystallographic information is available from OCA.
Reference
Solution structure of P22 transcriptional antitermination N peptide-boxB RNA complex., Cai Z, Gorin A, Frederick R, Ye X, Hu W, Majumdar A, Kettani A, Patel DJ, Nat Struct Biol. 1998 Mar;5(3):203-12. PMID:9501914
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