3mkj

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{{STRUCTURE_3mkj| PDB=3mkj | SCENE= }}
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==Methionine gamma-lyase from Citrobacter freundii with pyridoximine-5'-phosphate==
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===Methionine gamma-lyase from Citrobacter freundii with pyridoximine-5'-phosphate===
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<StructureSection load='3mkj' size='340' side='right' caption='[[3mkj]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20601224}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3mkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_freundii"_braak_1928 "bacterium freundii" braak 1928]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MKJ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PZP:[5-HYDROXY-4-(IMINOMETHYL)-6-METHYL-PYRIDIN-3-YL]METHYL+DIHYDROGEN+PHOSPHATE'>PZP</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2rfv|2rfv]], [[1y4i|1y4i]], [[3jwb|3jwb]], [[3jwa|3jwa]], [[3jw9|3jw9]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">megL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=546 "Bacterium freundii" Braak 1928])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_gamma-lyase Methionine gamma-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.11 4.4.1.11] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mkj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mkj RCSB], [http://www.ebi.ac.uk/pdbsum/3mkj PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/3mkj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pyridoxal 5'-phosphate (PLP) dependent methionine gamma-lyase catalyzes the breakdown of L-methionine to alpha-ketobutyric acid, methanethiol and ammonia. This enzyme, present in anaerobic microorganisms, has biomedical interest both for its activity as antitumor agent, depleting methionine supply in methionine-dependent cancers, and as target in the treatment of human pathogen infections, activating the pro-drug trifluoromethionine. To validate the structure of the enzyme from Citrobacter freundii, crystallized from monomethyl ether polyethylene glycol 2000, for the development of lead compounds, the reactivity of the crystalline enzyme towards L-methionine, substrate analogs and inhibitors was determined by polarized absorption microspectrophotometry. Spectral data were also collected for enzyme crystals, grown in monomethyl ether polyethylene glycol 2000 in the presence of ammonium sulfate. The three-dimensional structure of these enzyme crystals, solved at 1.65A resolution with R(free) 23.2%, revealed the surprising absence of the aldimine bond between the active site Lys210 and PLP. Different hypothesis are proposed and discussed in the light of spectral and structural data, pointing out to the relevance of the complementarity between X-ray crystallography and single crystal spectroscopy for the understanding of biological mechanisms at molecular level.
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==About this Structure==
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Exploring methionine gamma-lyase structure-function relationship via microspectrophotometry and X-ray crystallography.,Ronda L, Bazhulina NP, Morozova EA, Revtovich SV, Chekhov VO, Nikulin AD, Demidkina TV, Mozzarelli A Biochim Biophys Acta. 2010 Jul 1. PMID:20601224<ref>PMID:20601224</ref>
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[[3mkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MKJ OCA].
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==Reference==
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020601224</ref><references group="xtra"/><references/>
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</div>
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[[Category: Citrobacter freundii]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacterium freundii braak 1928]]
[[Category: Methionine gamma-lyase]]
[[Category: Methionine gamma-lyase]]
[[Category: Demidkina, T V.]]
[[Category: Demidkina, T V.]]

Revision as of 13:09, 18 May 2014

Methionine gamma-lyase from Citrobacter freundii with pyridoximine-5'-phosphate

3mkj, resolution 1.65Å

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