4nak

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{{STRUCTURE_4nak| PDB=4nak | SCENE= }}
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==Arabidopsis thaliana IspD in complex with pentabromo-pseudilin==
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===Arabidopsis thaliana IspD in complex with pentabromo-pseudilin===
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<StructureSection load='4nak' size='340' side='right' caption='[[4nak]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24446431}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4nak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NAK FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PBQ:PENTABROMOPSEUDILIN'>PBQ</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1w77|1w77]], [[2yc5|2yc5]], [[1ini|1ini]], [[4nai|4nai]], [[4nal|4nal]], [[4nan|4nan]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At2g02500, ISPD, MCT, MECT, MEPCT, T8K22.20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.60 2.7.7.60] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nak RCSB], [http://www.ebi.ac.uk/pdbsum/4nak PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzymes of the non-mevalonate pathway for isoprenoid biosynthesis have been identified as attractive targets with novel modes of action for the development of herbicides for crop protection and agents against infectious diseases. This pathway is present in many pathogenic organisms and plants, but absent in mammals. By using high-throughput screening, we identified highly halogenated marine natural products, the pseudilins, to be inhibitors of the third enzyme, IspD, in the pathway. Their activity against the IspD enzymes from Arabidopsis thaliana and Plasmodium vivax was determined in photometric and NMR-based assays. Cocrystal structures revealed that pseudilins bind to an allosteric pocket by using both divalent metal ion coordination and halogen bonding. The allosteric mode of action for preventing cosubstrate (CTP) binding at the active site was elucidated. Pseudilins show herbicidal activity in plant assays and antiplasmodial activity in cell-based assays.
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==Function==
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Pseudilins: Halogenated, Allosteric Inhibitors of the Non-Mevalonate Pathway Enzyme IspD.,Kunfermann A, Witschel M, Illarionov B, Martin R, Rottmann M, Hoffken HW, Seet M, Eisenreich W, Knolker HJ, Fischer M, Bacher A, Groll M, Diederich F Angew Chem Int Ed Engl. 2014 Jan 20. doi: 10.1002/anie.201309557. PMID:24446431<ref>PMID:24446431</ref>
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[[http://www.uniprot.org/uniprot/ISPD_ARATH ISPD_ARATH]] Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). Is essential for chloroplast development and required for pigments and gibberellins biosynthesis.<ref>PMID:10841550</ref> <ref>PMID:12029484</ref> <ref>PMID:18236010</ref>
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==About this Structure==
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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[[4nak]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAK OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:024446431</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase]]
[[Category: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase]]
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[[Category: Arath]]
[[Category: Bacher, A.]]
[[Category: Bacher, A.]]
[[Category: Diederich, F.]]
[[Category: Diederich, F.]]

Revision as of 06:48, 21 May 2014

Arabidopsis thaliana IspD in complex with pentabromo-pseudilin

4nak, resolution 1.80Å

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