3j67
From Proteopedia
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| - | + | ==Structural mechanism of the dynein powerstroke (post-powerstroke state)== | |
| - | + | <StructureSection load='3j67' size='340' side='right' caption='[[3j67]], [[Resolution|resolution]] 34.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3j67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strongylocentrotus_purpuratus Strongylocentrotus purpuratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J67 FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aki|4aki]], [[3j68|3j68]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j67 RCSB], [http://www.ebi.ac.uk/pdbsum/3j67 PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Dyneins are large microtubule motor proteins required for mitosis, intracellular transport and ciliary and flagellar motility. They generate force through a power-stroke mechanism, which is an ATP-consuming cycle of pre- and post-power-stroke conformational changes that cause relative motion between different dynein domains. However, key structural details of dynein's force generation remain elusive. Here, using cryo-electron tomography of intact, active (that is, beating), rapidly frozen sea urchin sperm flagella, we determined the in situ three-dimensional structures of all domains of both pre- and post-power-stroke dynein, including the previously unresolved linker and stalk of pre-power-stroke dynein. Our results reveal that the rotation of the head relative to the linker is the key action in dynein movement, and that there are at least two distinct pre-power-stroke conformations: pre-I (microtubule-detached) and pre-II (microtubule-bound). We provide three-dimensional reconstructions of native dyneins in three conformational states, in situ, allowing us to propose a molecular model of the structural cycle underlying dynein movement. | ||
| - | + | Structural mechanism of the dynein power stroke.,Lin J, Okada K, Raytchev M, Smith MC, Nicastro D Nat Cell Biol. 2014 Apr 13. doi: 10.1038/ncb2939. PMID:24727830<ref>PMID:24727830</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Strongylocentrotus purpuratus]] | [[Category: Strongylocentrotus purpuratus]] | ||
[[Category: Lin, J.]] | [[Category: Lin, J.]] | ||
Revision as of 06:55, 21 May 2014
Structural mechanism of the dynein powerstroke (post-powerstroke state)
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