1a7u
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1a7u.jpg|left|200px]] | + | [[Image:1a7u.jpg|left|200px]] |
| - | + | ||
| - | '''CHLOROPEROXIDASE T''' | + | {{Structure |
| + | |PDB= 1a7u |SIZE=350|CAPTION= <scene name='initialview01'>1a7u</scene>, resolution 1.75Å | ||
| + | |SITE= <scene name='pdbsite=CTA:Catalytic+Triad'>CTA</scene> and <scene name='pdbsite=CTB:Catalytic+Triad'>CTB</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] | ||
| + | |GENE= CPOT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 Streptomyces aureofaciens]) | ||
| + | }} | ||
| + | |||
| + | '''CHLOROPEROXIDASE T''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1A7U is a [ | + | 1A7U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7U OCA]. |
==Reference== | ==Reference== | ||
| - | Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:[http:// | + | Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9642069 9642069] |
[[Category: Chloride peroxidase]] | [[Category: Chloride peroxidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: peroxidase]] | [[Category: peroxidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:54:12 2008'' |
Revision as of 07:54, 20 March 2008
| |||||||
| , resolution 1.75Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Gene: | CPOT (Streptomyces aureofaciens) | ||||||
| Activity: | Chloride peroxidase, with EC number 1.11.1.10 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CHLOROPEROXIDASE T
Overview
The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. Based on these complexes and on the structure of an inactive variant, a reaction mechanism is proposed for the halogenation reaction with peroxoacid and hypohalous acid as reaction intermediates. Comparison of the structures suggests that a specific halide binding site is absent in the enzymes but that hydrophobic organic compounds may fit into the active site pocket for halogenation at preferential sites.
About this Structure
1A7U is a Single protein structure of sequence from Streptomyces aureofaciens. Full crystallographic information is available from OCA.
Reference
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:9642069
Page seeded by OCA on Thu Mar 20 09:54:12 2008
