1a9w
From Proteopedia
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| - | [[Image:1a9w.gif|left|200px]] | + | [[Image:1a9w.gif|left|200px]] |
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| - | '''HUMAN EMBRYONIC GOWER II CARBONMONOXY HEMOGLOBIN''' | + | {{Structure |
| + | |PDB= 1a9w |SIZE=350|CAPTION= <scene name='initialview01'>1a9w</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=CMO:CARBON MONOXIDE'>CMO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''HUMAN EMBRYONIC GOWER II CARBONMONOXY HEMOGLOBIN''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A9W is a [ | + | 1A9W is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A9W OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a human embryonic haemoglobin: the carbonmonoxy form of gower II (alpha2 epsilon2) haemoglobin at 2.9 A resolution., Sutherland-Smith AJ, Baker HM, Hofmann OM, Brittain T, Baker EN, J Mol Biol. 1998 Jul 17;280(3):475-84. PMID:[http:// | + | Crystal structure of a human embryonic haemoglobin: the carbonmonoxy form of gower II (alpha2 epsilon2) haemoglobin at 2.9 A resolution., Sutherland-Smith AJ, Baker HM, Hofmann OM, Brittain T, Baker EN, J Mol Biol. 1998 Jul 17;280(3):475-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9665850 9665850] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: oxygen transport]] | [[Category: oxygen transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:54:54 2008'' |
Revision as of 07:54, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN EMBRYONIC GOWER II CARBONMONOXY HEMOGLOBIN
Contents |
Overview
The production of recombinant embryonic haemoglobins via a yeast expression system has enabled structural and functional studies to be conducted on these proteins. As part of a programme aimed at understanding the properties of the embryonic haemoglobins we have crystallized the human alpha2 epsilon2 (Gower II) embryonic haemoglobin in its carbonmonoxy form, and determined its structure by X-ray crystallography. The structure was solved by molecular replacement and refined at 2.9 A to give a final model with R-factor=0.185 and Rfree=0.235. The Gower II hemoglobin tetramer is intermediate between the adult R and R2 states, though closer to R2. The tertiary structure of the conserved alpha subunit is essentially identical when compared to that found in the adult (alpha2 beta2) and fetal (alpha2 gamma2) hemoglobins. The embryonic epsilon subunit has a structure very similar to that of the homologous adult beta and fetal gamma subunits, although with small differences at the N terminus and in the A helix. Amino acid substitutions can be identified that may play a role in the altered response of the Gower II haemoglobin to allosteric effectors, in particular chloride ions. The reduced chloride effect is thought to be the primary cause of the higher affinity of this embryonic hemoglobin in comparison to the adult molecule.
Disease
Known diseases associated with this structure: Diphtheria, susceptibility to OMIM:[126150], Erythremias, alpha- OMIM:[141800], Erythrocytosis OMIM:[141850], Heinz body anemia OMIM:[141850], Heinz body anemias, alpha- OMIM:[141800], Hemoglobin H disease OMIM:[141850], Hypochromic microcytic anemia OMIM:[141850], Methemoglobinemias, alpha- OMIM:[141800], Thalassemia, alpha- OMIM:[141850], Thalassemias, alpha- OMIM:[141800]
About this Structure
1A9W is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a human embryonic haemoglobin: the carbonmonoxy form of gower II (alpha2 epsilon2) haemoglobin at 2.9 A resolution., Sutherland-Smith AJ, Baker HM, Hofmann OM, Brittain T, Baker EN, J Mol Biol. 1998 Jul 17;280(3):475-84. PMID:9665850
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