1aa6

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[[Image:1aa6.gif|left|200px]]<br /><applet load="1aa6" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1aa6.gif|left|200px]]
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caption="1aa6, resolution 2.3&Aring;" />
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'''REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br />
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{{Structure
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|PDB= 1aa6 |SIZE=350|CAPTION= <scene name='initialview01'>1aa6</scene>, resolution 2.3&Aring;
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|SITE= <scene name='pdbsite=4MO:The+Mo+Atom+Is+Coordinated+To+The+Se+Atom+Of+Sec+140+And+...'>4MO</scene> and <scene name='pdbsite=FS4:The+Fe+S+Cluster+Is+Coordinated+To+The+S+Atoms+Of+CYS+8,+...'>FS4</scene>
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|LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene> and <scene name='pdbligand=4MO:MOLYBDENUM(IV) ION'>4MO</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2]
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|GENE= FDHF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1AA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=MGD:'>MGD</scene> and <scene name='pdbligand=4MO:'>4MO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Known structural/functional Sites: <scene name='pdbsite=4MO:The+Mo+Atom+Is+Coordinated+To+The+Se+Atom+Of+Sec+140+And+...'>4MO</scene> and <scene name='pdbsite=FS4:The+Fe+S+Cluster+Is+Coordinated+To+The+S+Atoms+Of+CYS+8,+...'>FS4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA].
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1AA6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AA6 OCA].
==Reference==
==Reference==
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Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9036855 9036855]
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Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9036855 9036855]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Formate dehydrogenase]]
[[Category: Formate dehydrogenase]]
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[[Category: mpt]]
[[Category: mpt]]
[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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[[Category: secys]]
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[[Category: secy]]
[[Category: selenium]]
[[Category: selenium]]
[[Category: selenocysteine]]
[[Category: selenocysteine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:42:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:55:07 2008''

Revision as of 07:55, 20 March 2008

Image:1aa6.gif


PDB ID 1aa6

Drag the structure with the mouse to rotate
, resolution 2.3Å
Sites: and
Ligands: , and
Gene: FDHF (Escherichia coli)
Activity: Formate dehydrogenase, with EC number 1.2.1.2
Coordinates: save as pdb, mmCIF, xml



REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI


Overview

Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain alphabeta structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.

About this Structure

1AA6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of formate dehydrogenase H: catalysis involving Mo, molybdopterin, selenocysteine, and an Fe4S4 cluster., Boyington JC, Gladyshev VN, Khangulov SV, Stadtman TC, Sun PD, Science. 1997 Feb 28;275(5304):1305-8. PMID:9036855

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