1agt
From Proteopedia
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| - | [[Image:1agt.gif|left|200px]] | + | [[Image:1agt.gif|left|200px]] |
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| - | '''SOLUTION STRUCTURE OF THE POTASSIUM CHANNEL INHIBITOR AGITOXIN 2: CALIPER FOR PROBING CHANNEL GEOMETRY''' | + | {{Structure |
| + | |PDB= 1agt |SIZE=350|CAPTION= <scene name='initialview01'>1agt</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= AGTX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 Leiurus quinquestriatus hebraeus]) | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION STRUCTURE OF THE POTASSIUM CHANNEL INHIBITOR AGITOXIN 2: CALIPER FOR PROBING CHANNEL GEOMETRY''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AGT is a [ | + | 1AGT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGT OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the potassium channel inhibitor agitoxin 2: caliper for probing channel geometry., Krezel AM, Kasibhatla C, Hidalgo P, MacKinnon R, Wagner G, Protein Sci. 1995 Aug;4(8):1478-89. PMID:[http:// | + | Solution structure of the potassium channel inhibitor agitoxin 2: caliper for probing channel geometry., Krezel AM, Kasibhatla C, Hidalgo P, MacKinnon R, Wagner G, Protein Sci. 1995 Aug;4(8):1478-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8520473 8520473] |
[[Category: Leiurus quinquestriatus hebraeus]] | [[Category: Leiurus quinquestriatus hebraeus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: potassium channel blocker]] | [[Category: potassium channel blocker]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:57:20 2008'' |
Revision as of 07:57, 20 March 2008
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| Gene: | AGTX2 (Leiurus quinquestriatus hebraeus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE POTASSIUM CHANNEL INHIBITOR AGITOXIN 2: CALIPER FOR PROBING CHANNEL GEOMETRY
Overview
The structure of the potassium channel blocker agitoxin 2 was solved by solution NMR methods. The structure consists of a triple-stranded antiparallel beta-sheet and a single helix covering one face of the beta-sheet. The cysteine side chains connecting the beta-sheet and the helix form the core of the molecule. One edge of the beta-sheet and the adjacent face of the helix form the interface with the Shaker K+ channel. The fold of agitoxin is homologous to the previously determined folds of scorpion venom toxins. However, agitoxin 2 differs significantly from the other channel blockers in the specificity of its interactions. This study was thus focused on a precise characterization of the surface residues at the face of the protein interacting with the Shaker K+ channel. The rigid toxin molecule can be used to estimate dimensions of the potassium channel. Surface-exposed residues, Arg24, Lys27, and Arg31 of the beta-sheet, have been identified from mutagenesis studies as functionally important for blocking the Shaker K+ channel. The sequential and spatial locations of Arg24 and Arg31 are not conserved among the homologous toxins. Knowledge on the details of the channel-binding sites of agitoxin 2 formed a basis for site-directed mutagenesis studies of the toxin and the K+ channel sequences. Observed interactions between mutated toxin and channel are being used to elucidate the channel structure and mechanisms of channel-toxin interactions.
About this Structure
1AGT is a Single protein structure of sequence from Leiurus quinquestriatus hebraeus. Full crystallographic information is available from OCA.
Reference
Solution structure of the potassium channel inhibitor agitoxin 2: caliper for probing channel geometry., Krezel AM, Kasibhatla C, Hidalgo P, MacKinnon R, Wagner G, Protein Sci. 1995 Aug;4(8):1478-89. PMID:8520473
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