3mu7

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[[Image:3mu7.png|left|200px]]
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==Crystal structure of the xylanase and alpha-amylase inhibitor protein (XAIP-II) from scadoxus multiflorus at 1.2 A resolution==
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<StructureSection load='3mu7' size='340' side='right' caption='[[3mu7]], [[Resolution|resolution]] 1.29&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3mu7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scadoxus_multiflorus Scadoxus multiflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MU7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MU7 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hu7|3hu7]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mu7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mu7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mu7 RCSB], [http://www.ebi.ac.uk/pdbsum/3mu7 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/3mu7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Plants produce a wide range of proteinaceous inhibitors to protect themselves against hydrolytic enzymes. Recently a novel protein XAIP belonging to a new sub-family (GH18C) was reported to inhibit two structurally unrelated enzymes xylanase GH11 and alpha-amylase GH13. It was shown to inhibit xylanase GH11 with greater potency than that of alpha-amylase GH13. A new form of XAIP (XAIP-II) that inhibits alpha-amylase GH13 with a greater potency than that of XAIP and xylanase GH11 with a lower potency than that of XAIP, has been identified in the extracts of underground bulbs of Scadoxus multiflorus. This kind of occurrence of isoforms of inhibitor proteins is a rare observation and offers new opportunities for understanding the principles of protein engineering by nature. RESULTS: In order to determine the structural basis of the enhanced potency of XAIP-II against alpha-amylase GH13 and its reduced potency against xylanase GH11 as compared to that of XAIP, we have purified XAIP-II to homogeneity and obtained its complete amino acid sequence using cloning procedure. It has been crystallized with 0.1 M ammonium sulphate as the precipitating agent and the three-dimensional structure has been determined at 1.2 A resolution. The binding studies of XAIP-II with xylanase GH11 and alpha-amylase GH13 have been carried out with surface plasmon resonance (SPR). CONCLUSION: The structure determination revealed that XAIP-II adopts the well known TIM barrel fold. The xylanase GH11 binding site in XAIP-II is formed mainly with loop alpha3-beta3 (residues, 102 - 118) which has acquired a stereochemically less favorable conformation for binding to xylanase GH11 because of the addition of an extra residue, Ala105 and due to replacements of two important residues, His106 and Asn109 by Thr107 and Ser110. On the other hand, the alpha-amylase binding site, which consists of alpha-helices alpha6 (residues, 193 - 206), alpha7 (residues, 230 - 243) and loop beta6-alpha6 (residues, 180 - 192) adopts a stereochemically more favorable conformation due to replacements of residues, Ser190, Gly191 and Glu194 by Ala191, Ser192 and Ser195 respectively in alpha-helix alpha6, Glu231 and His236 by Thr232 and Ser237 respectively in alpha-helix alpha7. As a result, XAIP-II binds to xylanase GH11 less favorably while it interacts more strongly with alpha-amylase GH13 as compared to XAIP. These observations correlate well with the values of 4.2 x 10(-6) M and 3.4 x 10(-8) M for the dissociation constants of XAIP-II with xylanase GH11 and alpha-amylase GH13 respectively and those of 4.5 x 10(-7) M and 3.6 x 10(-6) M of XAIP with xylanase GH11 and alpha-amylase GH13 respectively.
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Modulation of inhibitory activity of xylanase-alpha-amylase inhibitor protein (XAIP): binding studies and crystal structure determination of XAIP-II from Scadoxus multiflorus at 1.2 A resolution.,Kumar S, Singh N, Mishra B, Dube D, Sinha M, Singh SB, Dey S, Kaur P, Sharma S, Singh TP BMC Struct Biol. 2010 Nov 20;10:41. PMID:21092126<ref>PMID:21092126</ref>
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The line below this paragraph, containing "STRUCTURE_3mu7", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_3mu7| PDB=3mu7 | SCENE= }}
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===Crystal structure of the xylanase and alpha-amylase inhibitor protein (XAIP-II) from scadoxus multiflorus at 1.2 A resolution===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_21092126}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 21092126 is the PubMed ID number.
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</StructureSection>
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-->
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{{ABSTRACT_PUBMED_21092126}}
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==About this Structure==
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[[3mu7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scadoxus_multiflorus Scadoxus multiflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MU7 OCA].
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==Reference==
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<ref group="xtra">PMID:021092126</ref><references group="xtra"/>
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[[Category: Scadoxus multiflorus]]
[[Category: Scadoxus multiflorus]]
[[Category: Kaur, P.]]
[[Category: Kaur, P.]]

Revision as of 10:34, 28 May 2014

Crystal structure of the xylanase and alpha-amylase inhibitor protein (XAIP-II) from scadoxus multiflorus at 1.2 A resolution

3mu7, resolution 1.29Å

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