3mji

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[[Image:3mji.png|left|200px]]
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==Activation of catalytic cysteine without a base in a Mutant Penicillin Acylase Precursor==
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<StructureSection load='3mji' size='340' side='right' caption='[[3mji]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3mji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MJI FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2pva|2pva]], [[3pva|3pva]], [[2quy|2quy]], [[2z71|2z71]], [[3mik|3mik]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mji OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mji RCSB], [http://www.ebi.ac.uk/pdbsum/3mji PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystallization of three catalytically inactive mutants of penicillin V acylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported. The mutant proteins crystallize in different primitive monoclinic space groups that are distinct from the crystal forms for the native enzyme. Directed mutants and clone constructs were designed to study the post-translational autoproteolytic processing of PVA. The catalytically inactive mutants will provide three-dimensional structures of precursor PVA forms, plus open a route to the study of enzyme-substrate complexes for this industrially important enzyme.
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Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants.,Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:16508111<ref>PMID:16508111</ref>
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The line below this paragraph, containing "STRUCTURE_3mji", creates the "Structure Box" on the page.
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3mji| PDB=3mji | SCENE= }}
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===Activation of catalytic cysteine without a base in a Mutant Penicillin Acylase Precursor===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_16508111}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 16508111 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_16508111}}
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==About this Structure==
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[[3mji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MJI OCA].
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==Reference==
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<ref group="xtra">PMID:016508111</ref><references group="xtra"/>
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[[Category: Lysinibacillus sphaericus]]
[[Category: Lysinibacillus sphaericus]]
[[Category: Penicillin amidase]]
[[Category: Penicillin amidase]]

Revision as of 10:36, 28 May 2014

Activation of catalytic cysteine without a base in a Mutant Penicillin Acylase Precursor

3mji, resolution 2.50Å

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