3faw

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{{Seed}}
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==Crystal Structure of the Group B Streptococcus Pullulanase SAP==
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[[Image:3faw.png|left|200px]]
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<StructureSection load='3faw' size='340' side='right' caption='[[3faw]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3faw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_coh1 Streptococcus agalactiae coh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FAW FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3fax|3fax]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAN_1346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342616 Streptococcus agalactiae COH1])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3faw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3faw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3faw RCSB], [http://www.ebi.ac.uk/pdbsum/3faw PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fa/3faw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The group B streptococcus type I pullulanase (SAP) is a class 13 glycoside hydrolase that is anchored to the bacterial cell surface via a conserved C-terminal anchoring motif and involved in alpha-glucan degradation. Recent in vitro functional studies have shown that SAP is immunogenic in humans and that anti-SAP sera derived from immunized animals impair both group A and group B streptococcus pullulanase activities, suggesting that in vivo immunization with this antigen could prevent streptococcal colonization. To further investigate the putative role of SAP in bacterial pathogenesis, we carried out functional studies and found that recombinant SAP binds to human cervical epithelial cells. Furthermore, with a view of using SAP as a vaccine candidate, we present high-resolution crystal structure analyses of an N-terminally truncated form of SAP lacking the carbohydrate binding module but containing the catalytic domain and displaying glycosidase hydrolase activity, both in its apo form and in complex with maltotetraose, at resolutions of 2.1 and 2.4 A, respectively.
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Group B streptococcus pullulanase crystal structures in the context of a novel strategy for vaccine development.,Gourlay LJ, Santi I, Pezzicoli A, Grandi G, Soriani M, Bolognesi M J Bacteriol. 2009 Jun;191(11):3544-52. Epub 2009 Mar 27. PMID:19329633<ref>PMID:19329633</ref>
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The line below this paragraph, containing "STRUCTURE_3faw", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_3faw| PDB=3faw | SCENE= }}
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===Crystal Structure of the Group B Streptococcus Pullulanase SAP===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<!--
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_19329633}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 19329633 is the PubMed ID number.
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</StructureSection>
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-->
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{{ABSTRACT_PUBMED_19329633}}
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==About this Structure==
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3FAW is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_coh1 Streptococcus agalactiae coh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FAW OCA].
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==Reference==
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<ref group="xtra">PMID:19329633</ref><references group="xtra"/>
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[[Category: Streptococcus agalactiae coh1]]
[[Category: Streptococcus agalactiae coh1]]
[[Category: Gourlay, L J.]]
[[Category: Gourlay, L J.]]
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[[Category: Secreted]]
[[Category: Secreted]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 10:22:14 2009''
 

Revision as of 10:42, 28 May 2014

Crystal Structure of the Group B Streptococcus Pullulanase SAP

3faw, resolution 2.10Å

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OCA

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