1aop
From Proteopedia
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| - | [[Image:1aop.jpg|left|200px]] | + | [[Image:1aop.jpg|left|200px]] |
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| - | '''SULFITE REDUCTASE STRUCTURE AT 1.6 ANGSTROM RESOLUTION''' | + | {{Structure |
| + | |PDB= 1aop |SIZE=350|CAPTION= <scene name='initialview01'>1aop</scene>, resolution 1.60Å | ||
| + | |SITE= <scene name='pdbsite=ACT:Four+Active+Site+Residues+That+Contact+The+Bound+Phosphate'>ACT</scene> and <scene name='pdbsite=BRG:CYS+Thiolate+In+The+Active+Center+That+Bridges+The+Heme+...'>BRG</scene> | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=SRM:SIROHEME'>SRM</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Sulfite_reductase_(NADPH) Sulfite reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.2 1.8.1.2] | ||
| + | |GENE= CYSIJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''SULFITE REDUCTASE STRUCTURE AT 1.6 ANGSTROM RESOLUTION''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AOP is a [ | + | 1AOP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1GEO. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOP OCA]. |
==Reference== | ==Reference== | ||
| - | Sulfite reductase structure at 1.6 A: evolution and catalysis for reduction of inorganic anions., Crane BR, Siegel LM, Getzoff ED, Science. 1995 Oct 6;270(5233):59-67. PMID:[http:// | + | Sulfite reductase structure at 1.6 A: evolution and catalysis for reduction of inorganic anions., Crane BR, Siegel LM, Getzoff ED, Science. 1995 Oct 6;270(5233):59-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7569952 7569952] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: snirr]] | [[Category: snirr]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:00:23 2008'' |
Revision as of 08:00, 20 March 2008
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| , resolution 1.60Å | |||||||
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| Sites: | and | ||||||
| Ligands: | , , and | ||||||
| Gene: | CYSIJ (Escherichia coli) | ||||||
| Activity: | Sulfite reductase (NADPH), with EC number 1.8.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SULFITE REDUCTASE STRUCTURE AT 1.6 ANGSTROM RESOLUTION
Overview
Fundamental chemical transformations for biogeochemical cycling of sulfur and nitrogen are catalyzed by sulfite and nitrite reductases. The crystallographic structure of Escherichia coli sulfite reductase hemoprotein (SiRHP), which catalyzes the concerted six-electron reductions of sulfite to sulfide and nitrite to ammonia, was solved with multiwavelength anomalous diffraction (MAD) of the native siroheme and Fe4S4 cluster cofactors, multiple isomorphous replacement, and selenomethionine sequence markers. Twofold symmetry within the 64-kilodalton polypeptide generates a distinctive three-domain alpha/beta fold that controls cofactor assembly and reactivity. Homology regions conserved between the symmetry-related halves of SiRHP and among other sulfite and nitrite reductases revealed key residues for stability and function, and identified a sulfite or nitrite reductase repeat (SNiRR) common to a redox-enzyme superfamily. The saddle-shaped siroheme shares a cysteine thiolate ligand with the Fe4S4 cluster and ligates an unexpected phosphate anion. In the substrate complex, sulfite displaces phosphate and binds to siroheme iron through sulfur. An extensive hydrogen-bonding network of positive side chains, water molecules, and siroheme carboxylates activates S-O bonds for reductive cleavage.
About this Structure
1AOP is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1GEO. Full crystallographic information is available from OCA.
Reference
Sulfite reductase structure at 1.6 A: evolution and catalysis for reduction of inorganic anions., Crane BR, Siegel LM, Getzoff ED, Science. 1995 Oct 6;270(5233):59-67. PMID:7569952[[Category: [4fe-4s]]]
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