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3qac

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[[Image:3qac.png|left|200px]]
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==Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.==
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<StructureSection load='3qac' size='340' side='right' caption='[[3qac]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3qac]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QAC FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3kgl|3kgl]], [[3ksc|3ksc]], [[2e9q|2e9q]], [[2d5f|2d5f]], [[2d5h|2d5h]], [[1od5|1od5]], [[1fxz|1fxz]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qac RCSB], [http://www.ebi.ac.uk/pdbsum/3qac PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a=b=97.6, c=74.8 A, gamma=120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a Vsol of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress.
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Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.,Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):919-22. Epub 2010 Jul 29. PMID:20693668<ref>PMID:20693668</ref>
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The line below this paragraph, containing "STRUCTURE_3qac", creates the "Structure Box" on the page.
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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{{STRUCTURE_3qac| PDB=3qac | SCENE= }}
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===Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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The line below this paragraph, {{ABSTRACT_PUBMED_20693668}}, adds the Publication Abstract to the page
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__TOC__
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(as it appears on PubMed at http://www.pubmed.gov), where 20693668 is the PubMed ID number.
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</StructureSection>
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{{ABSTRACT_PUBMED_20693668}}
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==About this Structure==
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[[3qac]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAC OCA].
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==Reference==
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<ref group="xtra">PMID:020693668</ref><references group="xtra"/>
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[[Category: Amaranthus hypochondriacus]]
[[Category: Amaranthus hypochondriacus]]
[[Category: Carrazco-Pena, L.]]
[[Category: Carrazco-Pena, L.]]

Revision as of 05:26, 4 June 2014

Structure of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.

3qac, resolution 2.27Å

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